Reduced expression of a distal gene of the prn gene cluster in deletion mutants of Aspergillus nidulans: genetic evidence for a dicistronic messenger in an eukaryote
- PMID: 355839
- DOI: 10.1007/BF00268959
Reduced expression of a distal gene of the prn gene cluster in deletion mutants of Aspergillus nidulans: genetic evidence for a dicistronic messenger in an eukaryote
Abstract
The prn gene cluster involved in L-proline catabolism in Aspergillus nidulans, has the gene order prnA-prnD-regulatory region-prnB-prnC. prnB, prnD, and prnC specify proline permease, proline oxidase, and delta1-pyrroline-5-carboxylate (P5C) dehydrogenase, respectively. prnA is probably a positive regulatory gene whose product is necessary for expression of the prn activities. Proline induces proline permease and P5C dehydrogenase in prnD- mutants which lack proline oxidase, showing that proline does not have to be converted to P5C to act as inducer. Deletion mutations extending from within prnD to within prnB result in considerably reduced expression of prnC, whereas a prnD- prnB- double mutant shows normal prnC expression. This strongly suggests that the deletion mutations eliminate a promotor/initiator site for transcription of a dicistronic messenger for prnB and prnC. The fact that the deletions do not eliminate prnC expression altogether indicates that at least one other species of prnC transcript (monocistronic, tricistronic, or tetracistronic) can be made.
Similar articles
-
The product of the regulatory gene of the proline catabolism gene cluster of Aspergillus nidulans is a positive-acting protein.Curr Genet. 1985;9(4):299-304. doi: 10.1007/BF00419959. Curr Genet. 1985. PMID: 3916725
-
Metabolite repression and inducer exclusion in the proline utilization gene cluster of Aspergillus nidulans.J Bacteriol. 2000 Jan;182(1):233-5. doi: 10.1128/JB.182.1.233-235.2000. J Bacteriol. 2000. PMID: 10613888 Free PMC article.
-
Gene roles in the prn cluster of Aspergillus nidulans.Curr Genet. 1981 Apr;3(1):49-56. doi: 10.1007/BF00419580. Curr Genet. 1981. PMID: 24189952
-
Regulation of levels of proline as an osmolyte in plants under water stress.Plant Cell Physiol. 1997 Oct;38(10):1095-102. doi: 10.1093/oxfordjournals.pcp.a029093. Plant Cell Physiol. 1997. PMID: 9399433 Review.
-
On the Evolution of Specificity in Members of the Yeast Amino Acid Transporter Family as Parts of Specific Metabolic Pathways.Int J Mol Sci. 2018 May 8;19(5):1398. doi: 10.3390/ijms19051398. Int J Mol Sci. 2018. PMID: 29738448 Free PMC article. Review.
Cited by
-
Two different, adjacent and divergent zinc finger binding sites are necessary for CREA-mediated carbon catabolite repression in the proline gene cluster of Aspergillus nidulans.EMBO J. 1994 Jan 15;13(2):407-15. doi: 10.1002/j.1460-2075.1994.tb06275.x. EMBO J. 1994. PMID: 8313886 Free PMC article.
-
The product of the regulatory gene of the proline catabolism gene cluster of Aspergillus nidulans is a positive-acting protein.Curr Genet. 1985;9(4):299-304. doi: 10.1007/BF00419959. Curr Genet. 1985. PMID: 3916725
-
Metabolite repression and inducer exclusion in the proline utilization gene cluster of Aspergillus nidulans.J Bacteriol. 2000 Jan;182(1):233-5. doi: 10.1128/JB.182.1.233-235.2000. J Bacteriol. 2000. PMID: 10613888 Free PMC article.
-
Do the tightly linked structural genes for nitrate and nitrite reductases in Aspergillus nidulans form an operon? Evidence from an insertional translocation which separates them.Mol Gen Genet. 1979 Jul 2;174(1):89-100. doi: 10.1007/BF00433309. Mol Gen Genet. 1979. PMID: 384164
-
Regulation of nitrogen metabolism and gene expression in fungi.Microbiol Rev. 1981 Sep;45(3):437-61. doi: 10.1128/mr.45.3.437-461.1981. Microbiol Rev. 1981. PMID: 6117784 Free PMC article. Review. No abstract available.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources