doi: 10.1007/BF00267410.
Lambda phage promoter used to enhance expression of a plasmid-cloned gene
- PMID: 355849
- DOI: 10.1007/BF00267410
Item in Clipboard
Lambda phage promoter used to enhance expression of a plasmid-cloned gene
Mol Gen Genet.
.
Abstract
A 50-fold (or greater) increase in the production of phage 21 repressor was obtained by construction of a plasmid in which the 21cI (repressor) gene could be transcribed from lambdaPL. The enhancement due to increased 21cI gene copy number and transcription from lambdaPL were at least five-fold and ten-fold, respectively. The plasmid was constructed in vitro by recombination of EcoRI-generated DNA fragments. The use of the DNA fragment containing lambdaPL in obtaining expression of cloned genes is discussed.
Similar articles
-
Construction of a hybrid bacteriophage-plasmid recombinant DNA vector.J Bacteriol. 1978 Dec;136(3):1192-6. doi: 10.1128/jb.136.3.1192-1196.1978. J Bacteriol. 1978. PMID: 363694 Free PMC article.
-
Cloning of the replication gene P of bacteriophage lambda: effects of increased P-protein synthesis on cellular and phage DNA replication.Mol Gen Genet. 1979 Mar 20;171(2):219-27. doi: 10.1007/BF00270008. Mol Gen Genet. 1979. PMID: 375032
-
DNA fusion product of phage P2 with plasmid pBR322: a new phasmid.Mol Gen Genet. 1983;189(2):343-7. doi: 10.1007/BF00337829. Mol Gen Genet. 1983. PMID: 6343796
-
DNA cloning vectors utilizing replication functions of the filamentous phages of Escherichia coli.J Gen Virol. 1986 Nov;67 ( Pt 11):2287-303. doi: 10.1099/0022-1317-67-11-2287. J Gen Virol. 1986. PMID: 3537204 Review. No abstract available.
-
The thirteenth Colworth Medal Lecture: The construction in vitro and exploitation of transducing derivatives of bacteriophage lambda.Biochem Soc Trans. 1977;5(6):1633-52. doi: 10.1042/bst0051633. Biochem Soc Trans. 1977. PMID: 340298 Review. No abstract available.
Cited by
-
Origin and binding specificity of protein(s) coded for by Mu prophages.Mol Gen Genet. 1979 Jun 7;173(2):189-96. doi: 10.1007/BF00330310. Mol Gen Genet. 1979. PMID: 158698
-
Studies on the E. coli groNB (nusB) gene which affects bacteriophage lambda N gene function.Mol Gen Genet. 1980;179(1):55-61. doi: 10.1007/BF00268446. Mol Gen Genet. 1980. PMID: 6161293
-
Strategies for achieving high-level expression of genes in Escherichia coli.Microbiol Rev. 1996 Sep;60(3):512-38. doi: 10.1128/mr.60.3.512-538.1996. Microbiol Rev. 1996. PMID: 8840785 Free PMC article. Review.
-
DNA sequence encoding the NH2-terminal peptide involved in transport of lambda receptor, an Escherichia coli secretory protein.Proc Natl Acad Sci U S A. 1980 May;77(5):2621-5. doi: 10.1073/pnas.77.5.2621. Proc Natl Acad Sci U S A. 1980. PMID: 6446717 Free PMC article.
-
Phase variation and the Hin protein: in vivo activity measurements, protein overproduction, and purification.J Bacteriol. 1984 Jul;159(1):71-9. doi: 10.1128/jb.159.1.71-79.1984. J Bacteriol. 1984. PMID: 6330051 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
