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. 1978 Jul 11;163(2):197-203.
doi: 10.1007/BF00267410.

Lambda phage promoter used to enhance expression of a plasmid-cloned gene

J Hedgpeth, M Ballivet, H Eisen

Lambda phage promoter used to enhance expression of a plasmid-cloned gene

J Hedgpeth et al. Mol Gen Genet. .

Abstract

A 50-fold (or greater) increase in the production of phage 21 repressor was obtained by construction of a plasmid in which the 21cI (repressor) gene could be transcribed from lambdaPL. The enhancement due to increased 21cI gene copy number and transcription from lambdaPL were at least five-fold and ten-fold, respectively. The plasmid was constructed in vitro by recombination of EcoRI-generated DNA fragments. The use of the DNA fragment containing lambdaPL in obtaining expression of cloned genes is discussed.

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