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. 2022 Aug;64(8):1514-1530.
doi: 10.1111/jipb.13281. Epub 2022 Jun 13.

PP2A interacts with KATANIN to promote microtubule organization and conical cell morphogenesis

Huibo Ren  1 , Jinqiu Rao  1 , Min Tang  1 , Yaxing Li  1 , Xie Dang  1 , Deshu Lin  1   2
Affiliations

PP2A interacts with KATANIN to promote microtubule organization and conical cell morphogenesis

Huibo Ren et al. J Integr Plant Biol. 2022 Aug.

Abstract

The organization of the microtubule cytoskeleton is critical for cell and organ morphogenesis. The evolutionarily conserved microtubule-severing enzyme KATANIN plays critical roles in microtubule organization in the plant and animal kingdoms. We previously used conical cell of Arabidopsis thaliana petals as a model system to investigate cortical microtubule organization and cell morphogenesis and determined that KATANIN promotes the formation of circumferential cortical microtubule arrays in conical cells. Here, we demonstrate that the conserved protein phosphatase PP2A interacts with and dephosphorylates KATANIN to promote the formation of circumferential cortical microtubule arrays in conical cells. KATANIN undergoes cycles of phosphorylation and dephosphorylation. Using co-immunoprecipitation coupled with mass spectrometry, we identified PP2A subunits as KATANIN-interacting proteins. Further biochemical studies showed that PP2A interacts with and dephosphorylates KATANIN to stabilize its cellular abundance. Similar to the katanin mutant, mutants for genes encoding PP2A subunits showed disordered cortical microtubule arrays and defective conical cell shape. Taken together, these findings identify PP2A as a regulator of conical cell shape and suggest that PP2A mediates KATANIN phospho-regulation during plant cell morphogenesis.

Keywords: KATANIN; conical cells; cortical microtubules; morphogenesis; phospho-regulation; protein phosphatase PP2A.

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References

REFERENCES

    1. Adamowski, M., and Friml, J. (2015). PIN-dependent auxin transport: Action, regulation, and evolution. Plant Cell 27: 20-32.
    1. Bian, C., Guo, X., Zhang, Y., Wang, L., Xu, T., DeLong, A., and Dong, J. (2020). Protein phosphatase 2A promotes stomatal development by stabilizing SPEECHLESS in Arabidopsis. Proc. Natl. Acad. Sci. U.S.A. 117: 13127-13137.
    1. Baskin, T.I. (2001). On the alignment of cellulose microfibrils by cortical microtubules: A review and a model. Protoplasma 215: 150-171.
    1. Bichet, A., Desnos, T., Turner, S., Grandjean, O., and Höfte, H. (2001). BOTERO1 is required for normal orientation of cortical microtubules and anisotropic cell expansion in Arabidopsis. Plant J. 25: 137-148.
    1. Blakeslee, J.J., Zhou, H.W., Heath, J.T., Skottke, K.R., Barrios, J.A., Liu, S.Y., and DeLong, A. (2008). Specificity of RCN1-mediated protein phosphatase 2A regulation in meristem organization and stress response in roots. Plant Physiol. 146: 539-553.

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