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. 2022 Sep;65(9):459-465.
doi: 10.3345/cep.2022.00234. Epub 2022 May 19.

Plastic bottle feeding produces changes in biochemical parameters in human infants - A pilot study

Mahendra K Pant  1 , Abul H Ahmad  2 , Manisha Naithani  3 , Jayanti Pant  4
Affiliations

Plastic bottle feeding produces changes in biochemical parameters in human infants - A pilot study

Mahendra K Pant et al. Clin Exp Pediatr. 2022 Sep.

Abstract

Background: Plastic bottles are widely used by people to feed their infants when breastfeeding is not possible. Bisphenol A (BPA), an endocrine disruptor is widely used in the manufacturing of plastic wares and is leached out from these plastic wares on exposure to high temperature, changed pH, or cleaning the plastic wares by harsh detergents.

Purpose: Feeding through plastic bottles over prolong duration is expected to expose the infants to leached BPA. Hence the present study was taken up to compare the effects of breastfeeding and plastic bottle feeding on biochemical parameters in infants and also detect for the presence of free BPA or its metabolite in the infants.

Methods: Biochemical tests like lipid profile, liver function tests, creatine-kinase-MB (CK-MB), serum urea, serum electrolytes were performed on blood samples obtained from infants who were breastfed and plastic bottle fed. Further, plasma and urine samples of the infants were subjected to Liquid chromatography-mass spectrometry analysis for detecting free BPA and BPA glucuronide.

Results: Biochemical changes in form of raised triglycerides, cholesterol, low-density lipoproteins, very low-density lipoproteins and increase in CK-MB, serum urea were observed in plastic bottle fed infants. BPA glucuronide was also detected in the urine of these infants. Free BPA was not detected in plasma or urine samples of the infants except in one plasma sample from bottle-fed group.

Conclusion: Plastic bottle feeding may lead to toxic changes in the functioning of organs which manifest as altered biochemical parameters.

Keywords: Bottle feeding; Breast feeding/methods; Chromatography; Infants; Liquid.

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Conflict of interest statement

Conflicts of interest

No potential conflict of interest relevant to this article was reported.

Figures

Fig. 1.
Fig. 1.
Schema for plasma and urine sample collection and use in different tests. LCMS/MS, liquid chromatography-mass spectrometry; BPA, bisphenol A; HPLC, high performance liquid chromatography.
Fig. 2.
Fig. 2.
Comparison of biochemical parameters between breastfed infants and bottle-fed infants. LDL, lowdensity lipoprotein; VLDL, very low-density lipoprotein; CK-MB, creatine-kinase–MB.
Fig. 3.
Fig. 3.
Bisphenol A glucuronide detected in urine of infants through liquid chromatography-mass spectrometry analysis. TOF, time of flight; MS, mass spectrometer; TIC, total ion chromatography.
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