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Review
. 2022 Oct:59:107983.
doi: 10.1016/j.biotechadv.2022.107983. Epub 2022 May 16.

Boosting extracellular vesicle secretion

Lior Debbi  1 , Shaowei Guo  2 , Dina Safina  1 , Shulamit Levenberg  3
Affiliations
Review

Boosting extracellular vesicle secretion

Lior Debbi et al. Biotechnol Adv. 2022 Oct.

Abstract

In recent years, extracellular vesicles (EVs), specifically exosomes, have emerged as a promising strategy for treating a wide spectrum of pathologies, such as cancer and COVID-19, as well as promoting tissue regeneration in various conditions, including cardiomyopathies and spinal cord injuries. Despite the great potential of EV-based therapies, poor yield and unscalable production of EVs remain big challenges to overcome to translate these types of treatment to clinical practices. Here, we review different strategies for enhancing EV yield by physical, biological or chemical means. Some of these novel approaches can lead to about 100-fold increase in EV production yield, thus bringing closer the clinical translation with regard to scalability and efficiency.

Keywords: Drug delivery; Exosomes; Extracellular vesicles; Large-scale.

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Figures

Fig. 1
Fig. 1
Novel strategies for boosting extracellular vesicle secretion. EV secretion rate can be significantly enhanced up to two orders of magnitude using various triggers including physical signals, molecular interference, environmental factors, and external inducers.
See this image and copyright information in PMC

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