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. 2022 Apr 30;20(1):15593258221088709.
doi: 10.1177/15593258221088709. eCollection 2022 Jan-Mar.

Green Synthesis of Silver Nanoparticles (AgNPs), Structural Characterization, and their Antibacterial Potential

Muhammad Asif  1 , Riffat Yasmin  2 , Rizwan Asif  3 , Ana Ambreen  3 , Madiha Mustafa  3 , Shehla Umbreen  3
Affiliations

Green Synthesis of Silver Nanoparticles (AgNPs), Structural Characterization, and their Antibacterial Potential

Muhammad Asif et al. Dose Response. .

Abstract

Background: In the field of nanotechnology, the metallic nanoparticles are of remarkable interest because of their unique electronic, magnetic, chemical, and mechanical properties.

Purpose: In the present work, silver nanoparticles (AgNPs) were synthesized using bio-reduction method.

Research design: Silver nitrate was used as metallic precursor and the extract of Moringa oleifera leaves with different concentrations was used as reducing as well capping agent. The extract exhibited strong potential in rapid reduction of silver ions for the synthesis of silver nanoparticles. The synthesized silver nanoparticles were characterized by UV-visible spectroscopy, X-ray diffraction (XRD), and scanning electron microscopy (SEM) techniques.

Results: The absorption SPR peaks appeared in the range of 415 to 439 nm. SEM analysis exhibited that particles were spherical in shape with size distribution range from 10 nm to 25 nm. The synthesized silver nanoparticles were pure crystalline in nature as confirmed by the XRD spectra with average crystallite size 7 nm. In vitro antibacterial activity of the prepared silver nanoparticles colloidal samples as well the extract was studied using different concentrations of AgNPs (C1 = 100 μg/ml, C2 = 50 μg/ml, C3 = 25 μg/ml) by well diffusion method against Gram negative Escherichia coli. The antibacterial performance was assessed by measuring the zone of inhibition (ZOI).

Conclusions: The results suggested that AgNPs prepared by green approach can be considered as an alternative antibacterial agent.

Keywords: AgNPs; Escherichia coli; Moringa oleifera; antibacterial agent; green synthesis.

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Conflict of interest statement

Declaration of Conflicting Interests: The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Figures

Figure 1.
Figure 1.
Extract from M. oleifera leaves.
Figure 2.
Figure 2.
Synthesis of silver nanoparticles using M. oleifera leaves extract.
Figure 3.
Figure 3.
Four different types of AgNPs shown different colors along with extract and AgNO3.
Figure 4.
Figure 4.
UV-Vis absorption spectra of silver nanoparticles AgNO3 and extract.
Figure 5.
Figure 5.
X-ray diffraction spectra of M. oleifera leaf extract.
Figure 6.
Figure 6.
(a) SEM image of AgNPs, (b) Particular section magnified image (c) Particle size distribution histogram.
Figure 7.
Figure 7.
(a and b) Antibacterial activities of AgNPs against E. coli. (c) ZOI histogram.
Figure 8.
Figure 8.
Effect of different concentrations of (MLE-AgNPs) on the growth of E. coli.
Figure 9.
Figure 9.
Effect of (MLE-AgNPs) on the growth of E. coli at different time interval.
See this image and copyright information in PMC

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