Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 May 21;33(6):46.
doi: 10.1007/s10856-022-06666-3.

Improving bactericidal performance of implant composite coatings by synergism between Melittin and tetracycline

Vahid Zarghami  1 , Mohammad Ghorbani  2   3 , Kamran Pooshang Bagheri  4 , Mohammad Ali Shokrgozar  5
Affiliations

Improving bactericidal performance of implant composite coatings by synergism between Melittin and tetracycline

Vahid Zarghami et al. J Mater Sci Mater Med. .

Abstract

Methicillin resistance Staphylococcus aureus bacteria (MRSA) are serious hazards of bone implants. The present study was aimed to use the potential synergistic effects of Melittin and tetracycline to prevent MRSA associated bone implant infection. Chitosan/bioactive glass nanoparticles/tetracycline composite coatings were deposited on hydrothermally etched titanium substrate. Melittin was then coated on composite coatings by drop casting method. The surfaces were analyzed by FTIR, XRD, and SEM instruments. Tetracycline in coatings revealed multifunctional behaviors include bone regeneration and antibacterial activity. Releasing ALP enzyme from MC3T3 cells increased by tetracycline, so it is suitable candidate as osteoinductive and antibacterial agent in orthopedic implants coatings. Melittin increased the proliferation of MC3T3 cells. Composite coatings with combination of tetracycline and Melittin eradicate all MRSA bacteria, while coatings with one of them could no t eradicate all of the bacteria. In conclusion, chitosan/bioactive glass/tetracycline/Melittin coating can be suggested as a multifunctional bone implant coating because of its osteogenic and promising antibacterial activity. Graphical abstract.

PubMed Disclaimer

Conflict of interest statement

The authors declare no competing interests.

Figures

None
Graphical abstract
Fig. 1
Fig. 1
A FE-SEM images of biomedical grade Titanium sheets after hydrothermal etching at 10 kx and 50 kx magnification. B Surface roughness changes. C GIXRD pattern of hydrothermally etched Ti
Fig. 2
Fig. 2
FE-SEM images of CS, CBG, CBTE, CBME and CBTME coatings (SEM magnification in all images is 10.0 kx)
Fig. 3
Fig. 3
FTIR spectra of pristine components and composite coatings
Fig. 4
Fig. 4
Alamar blue assay of experimental groups after 1 and 7 days (*P < 0.05, **P < 0.01, ***P < 0.001)
Fig. 5
Fig. 5
Fluorescence images after live/dead staining of MC3T3 cells cultured for 2 days on experimental groups
Fig. 6
Fig. 6
Normalized ALP assays of MC3T3 cells cultured on the surfaces after 5 and 10 days (*P < 0.05, **P < 0.01, ***P < 0.001)
Fig. 7
Fig. 7
A The numbers of planktonic bacteria. B The numbers of adhered bacteria on different surfaces (*P < 0.05, **P < 0.01, ***P < 0.001)
Fig. 8
Fig. 8
SEM images of MRSA bacteria on experimental groups after incubation for 6 h (SEM magnification in all images is 30.0 kx)
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Liu Z, Liu X, Ramakrishna S. Surface engineering of biomaterials in orthopedic and dental implants: Strategies to improve osteointegration, bacteriostatic and bactericidal activities. Biotechnol J. 2021;16:2000116. doi: 10.1002/biot.202000116. - DOI - PubMed
    1. Chen J, Hu G, Li T, Chen Y, Gao M, Li Q, et al. Fusion peptide engineered “statically-versatile” titanium implant simultaneously enhancing anti-infection, vascularization and osseointegration. Biomaterials. 2021;264:120446. doi: 10.1016/j.biomaterials.2020.120446. - DOI - PubMed
    1. Ghilini F, Fagali N, Pissinis DE, Benítez G, Schilardi PL. Multifunctional titanium surfaces for orthopedic implants: antimicrobial activity and enhanced osseointegration. ACS Appl Bio Mater. 2021;4:6451–61. doi: 10.1021/acsabm.1c00613. - DOI - PubMed
    1. Wang B, Bian A, Jia, F, Lan J, Yang H, Yan K, et al. (2022) “Dual-functional” strontium titanate nanotubes designed based on fusion peptides simultaneously enhancing anti-infection and osseointegration. Mater Sci & Eng C. p.112650. 10.1016/j.msec.2022.112650 - PubMed
    1. Moses, JC, Mandal BB (2022) Mesoporous silk-bioactive glass nanocomposites as drug eluting multifunctional conformal coatings for improving osseointegration and bactericidal properties of metal implants. ACS Appl Mater & Interf. 10.1021/acsami.2c00093 - PubMed

MeSH terms