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. 2022 May 5:13:883653.
doi: 10.3389/fphar.2022.883653. eCollection 2022.

Neutral Polysaccharides From Hohenbuehelia serotina With Hypoglycemic Effects in a Type 2 Diabetic Mouse Model

Qinghong Liu  1 , Jing Wu  2 , Peng Wang  1 , Yuxiao Lu  3 , Xinhe Ban  4
Affiliations

Neutral Polysaccharides From Hohenbuehelia serotina With Hypoglycemic Effects in a Type 2 Diabetic Mouse Model

Qinghong Liu et al. Front Pharmacol. .

Abstract

Neutral polysaccharides (NHSPs) from the mushroom Hohenbuehelia serotina were purified by D301/D152 resin ion-exchange chromatography and DEAE-cellulose anion exchange chromatography. The weight-average molecular weight (MW) and number-average molecular weight (Mn) of NHSP were 1,821 and 820.55 kDa, respectively. A monosaccharide component analysis showed that NHSP was composed of glucose, galactose, and mannose in molar ratio 2.6:2.1:1.0. FT-IR and NMR (1H and HSQC) spectroscopic analyses revealed that NHSP contained mainly 1,3-linked β-D-glucose, 1,4-linked β-D-glucose, 1,6-linked β-D-mannose, 1,6-linked α-D-mannose, and 1,6-linked β-D-galactose. The thermogravimetric analysis (TGA) showed that NHSP has good thermal stability below 250°C. NHSP notably reduced the blood glucose level (hypoglycemic effect) at dose 200 mg/kg for 21 days in a type 2 diabetic mouse model. NHSP reduced the liver index significantly, suggesting that it may help prevent hepatic steatosis or hepatomegaly.

Keywords: Hohenbuehelia serotina; hypoglycemic effect; neutral polysaccharide; structural characterization; type 2 diabetes.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
Purification scheme of Hohenbuehelia serotina polysaccharides.
FIGURE 2
FIGURE 2
FT-IR spectra of NHSP.
FIGURE 3
FIGURE 3
1H NMR spectra (A) (in D2O) and HSQC spectra (B) of NHSP.
FIGURE 4
FIGURE 4
Weight loss percentage and derivative thermogravimetric curves of NHSP.
FIGURE 5
FIGURE 5
Scanning electron micrographs of NHSP [(A): ×100; (B) ×500; (C) ×1,000; and (D) ×2000].
FIGURE 6
FIGURE 6
Effects of NHSP treatment on a type 2 diabetic mouse model. (A) Effect of NHSP on weight. (B) Effect of NHSP on FBG. Values are presented as mean ± S.D. *p < 0.05 for comparison with MC group. **p < 0.01 for comparison with MC group.
See this image and copyright information in PMC

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