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. 2022 May;13(5):12289-12297.
doi: 10.1080/21655979.2022.2070965.

Long non-coding RNA HLA-F antisense RNA 1 inhibits the maturation of microRNA-613 in polycystic ovary syndrome to promote ovarian granulosa cell proliferation and inhibit cell apoptosis

Xiaohua Li  1 , Laifang Zhu  1 , Yan Luo  1
Affiliations

Long non-coding RNA HLA-F antisense RNA 1 inhibits the maturation of microRNA-613 in polycystic ovary syndrome to promote ovarian granulosa cell proliferation and inhibit cell apoptosis

Xiaohua Li et al. Bioengineered. 2022 May.

Abstract

MicroRNA-613 (miR-613) inhibits granulosa cell proliferation, suggesting its involvement in polycystic ovary syndrome (PCOS). We predicted that long non-coding RNA (lncRNA) HLA-F antisense RNA 1 (HLA-F-AS1) could interact with premature miR-613. We then explored the crosstalk between HLA-F-AS1 and miR-613 in PCOS. In this study, follicular fluid donated by 58 healthy controls and 58 PCOS patients was used to analyze the expression of HLA-F-AS1 and miR-613 (mature and premature). The direct interaction between HLA-F-AS1 and premature miR-613 was evaluated by RNA pull-down assay. Overexpression of both HLA-F-AS1 and miR-613 was achieved in granulosa cells to assess their interactions. Cell proliferation and apoptosis were detected with BrdU assay and cell apoptosis assay, respectively. We found that miR-613 was highly expressed in PCOS, while HLA-F-AS1 was downregulated in PCOS. HLA-F-AS1 directly interacted with premature miR-613, and overexpression of HLA-F-AS1 increased the expression levels of premature miR-613, but decreased the expression levels of mature miR-613. HLA-F-AS1 increased ovarian granulosa cell proliferation and inhibited cell apoptosis. MiR-613 played an opposite role and suppressed the role of HLA-F-AS1. Therefore, HLA-F-AS1 may inhibit the maturation of miR-613 in PCOS to promote ovarian granulosa cell proliferation and inhibit cell apoptosis.

Keywords: HLA-F-AS1; Polycystic ovary syndrome; apoptosis; miR-613; proliferation.

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Conflict of interest statement

No potential conflict of interest was reported by the author(s).

Figures

None
Graphical abstract
Figure 1.
Figure 1.
Analysis of the expression of HLA-F-AS1 and miR-613 in PCOS.
Figure 2.
Figure 2.
Correlations between HLA-F-AS1 and miR-613 across PCOS samples.
Figure 3.
Figure 3.
Exploration of the direct interaction of HLA-F-AS1 with premature miR-613, and the detection of HLA-F-AS1 in subcellular location of COV434 cells.
Figure 4.
Figure 4.
Analysis of the role of HLA-F-AS1 in the maturation of miR-613.
Figure 5.
Figure 5.
Analysis of the role of HLA-F-AS1 and miR-613 in the proliferation and apoptosis of COV434 cells.
See this image and copyright information in PMC

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