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. 2022 Jul:62:102815.
doi: 10.1016/j.scr.2022.102815. Epub 2022 May 17.

Generation of isogenic control DJ-1-delP GC13 for the genetic Parkinson's disease-patient derived iPSC line DJ-1-delP (LCSBi008-A-1)

Pauline Mencke  1 , Zoé Hanss  1 , Javier Jarazo  2 , François Massart  1 , Arkadiusz Rybicki  1 , Elizabet Petkovski  3 , Enrico Glaab  4 , Ibrahim Boussaad  1 , Vincenzo Bonifati  5 , Jens Christian Schwamborn  2 , Wim Mandemakers  5 , Rejko Krüger  6
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Generation of isogenic control DJ-1-delP GC13 for the genetic Parkinson's disease-patient derived iPSC line DJ-1-delP (LCSBi008-A-1)

Pauline Mencke et al. Stem Cell Res. 2022 Jul.
Free article

Abstract

We describe the generation of an isogenic control cell line DJ-1-delP GC13 from an induced pluripotent stem cell (iPSC) line DJ-1-delP LCSBi008-A that was derived from fibroblasts obtained from a Parkinson's disease (PD) patient. Using CRISPR/Cas9 technology, we corrected the disease causing c.471_473delGCC homozygous mutation in the PARK7 gene leading to p.158P deletion in the encoded protein DJ-1. The generated isogenic pair will be used for phenotypic analysis of PD-patient derived neurons and astrocytes.

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