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. 2022:2453:261-278.
doi: 10.1007/978-1-0716-2115-8_15.

AIRR Community Guide to Planning and Performing AIRR-Seq Experiments

Anne Eugster  1 , Magnolia L Bostick  2   3 , Nidhi Gupta  2 , Encarnita Mariotti-Ferrandiz  4 , Gloria Kraus  1 , Wenzhao Meng  5 , Cinque Soto  6 , Johannes Trück  7 , Ulrik Stervbo  8 , Eline T Luning Prak  9 ; AIRR Community
Affiliations

AIRR Community Guide to Planning and Performing AIRR-Seq Experiments

Anne Eugster et al. Methods Mol Biol. 2022.

Abstract

The development of high-throughput sequencing of adaptive immune receptor repertoires (AIRR-seq of IG and TR rearrangements) has provided a new frontier for in-depth analysis of the immune system. The last decade has witnessed an explosion in protocols, experimental methodologies, and computational tools. In this chapter, we discuss the major considerations in planning a successful AIRR-seq experiment together with basic strategies for controlling and evaluating the outcome of the experiment. Members of the AIRR Community have authored several chapters in this edition, which cover step-by-step instructions to successfully conduct, analyze, and share an AIRR-seq project.

Keywords: AIRR-seq; Antibody; Immune repertoire; Immunoglobulin; Next-generation sequencing; T-cell receptor; V(D)J recombination.

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References

    1. Boudinot P, Marriotti-Ferrandiz ME, Pasquier LD, Benmansour A, Cazenave PA, Six A (2008) New perspectives for large-scale repertoire analysis of immune receptors. Mol Immunol 45(9):2437–2445. https://doi.org/10.1016/j.molimm.2007.12.018 doi: 10.1016/j.molimm.2007.12.018. - DOI - PubMed
    1. Robins HS, Campregher PV, Srivastava SK, Wacher A, Turtle CJ, Kahsai O et al (2009) Comprehensive assessment of T-cell receptor beta-chain diversity in alphabeta T cells. Blood 114(19):4099–4107. https://doi.org/10.1182/blood-2009-04-217604 doi: 10.1182/blood-2009-04-217604. - DOI - PMC - PubMed
    1. Georgiou G, Ippolito GC, Beausang J, Busse CE, Wardemann H, Quake SR (2014) The promise and challenge of high-throughput sequencing of the antibody repertoire. Nat Biotechnol 32(2):158–168. https://doi.org/10.1038/nbt.2782 doi: 10.1038/nbt.2782. - DOI - PMC - PubMed
    1. Breden F, Luning Prak ET, Peters B, Rubelt F, Schramm CA, Busse CE et al (2017) Reproducibility and reuse of adaptive immune receptor repertoire data. Front Immunol 8:1418. https://doi.org/10.3389/fimmu.2017.01418 doi: 10.3389/fimmu.2017.01418. - DOI - PMC - PubMed
    1. Tonegawa S (1983) Somatic generation of antibody diversity. Nature 302(5909):575–581. https://doi.org/10.1038/302575a0 doi: 10.1038/302575a0. - DOI - PubMed

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