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. 2022 Mar 30;71(1):91-105.
doi: 10.33073/pjm-2022-012.

Evaluation of the Probiotic Potential of Lactobacillus delbrueckii ssp. indicus WDS-7 Isolated from Chinese Traditional Fermented Buffalo Milk In Vitro

Changjun Wu  1 , Chenwei Dai  1 , Lin Tong  1 , Han Lv  1 , Xiuhong Zhou  1
Affiliations

Evaluation of the Probiotic Potential of Lactobacillus delbrueckii ssp. indicus WDS-7 Isolated from Chinese Traditional Fermented Buffalo Milk In Vitro

Changjun Wu et al. Pol J Microbiol. .

Abstract

The present study aimed to evaluate the probiotic potential of lactic acid bacteria (LAB) isolated from Chinese traditional fermented buffalo milk. Out of 22 isolates, 11 were putatively identified as LAB preliminarily. A total of six LAB strains displayed strong adhesion to HT-29 cells and all these strains showed preferable tolerance to artificially simulated gastrointestinal juices. WDS-4, WDS-7, and WDS-18 exhibited excellent antioxidant capacities, including DPPH radical, ABTS+ radical, and superoxide anion scavenging activities. Compared with the other two LAB strains, WDS-7 had a stronger inhibition effect on four pathogens. Based on the 16S rRNA gene sequencing and phylogenetic analysis, WDS-7 was identified as Lactobacillus delbrueckii ssp. indicus and selected to assess the potential and safety of probiotics further. The results revealed that WDS-7 strain had a strong capacity for acid production and good thermal stability. WDS-7 strain also possessed bile salt hydrolase (BSH) activity. Compared to LGG, WDS-7 was a greater biofilm producer on the plastic surface and exhibited a better EPS production ability (1.94 mg/ml as a glucose equivalent). WDS-7 was proved to be sensitive in the majority of tested antibiotics and absence of hemolytic activity. Moreover, no production of biogenic amines and β-glucuronidase was observed in WDS-7. The findings of this work indicated that L. delbrueckii ssp. indicus WDS-7 fulfilled the probiotic criteria in vitro and could be exploited for further evaluation in vivo.

Keywords: Lactobacillus delbrueckii ssp. indicus; fermented buffalo milk; food application; lactic acid bacteria; probiotic potential.

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Conflict of interest statement

Conflict of interest

The authors do not report any financial or personal connections with other persons or organizations, which might negatively affect the contents of this publication and/or claim authorship rights to this publication.

Figures

Fig. 1
Fig. 1
Antibacterial activity of six isolated LAB strains. a–c) Different letters represent statistical significance (p < 0.05).
Fig. 2
Fig. 2
Phylogenetic tree constructed based on the 16S rRNA gene sequence of Lactobacillus delbrueckii ssp. indicus WDS-7 strain.
Fig. 3
Fig. 3
SEM images of Lactobacillus delbrueckii ssp. indicus WDS-7 strain. A) SEM image of numerous bacteria located at random, B) SEM image of a single cell.
Fig. 4
Fig. 4
Growth curve and acid production curve of Lactobacillus delbrueckii ssp. indicus WDS-7 strain.
Fig. 5
Fig. 5
EPS production activity of Lactobacillus delbrueckii ssp. indicus WDS-7 strain. * – represents the statistical significance (p < 0.05).
See this image and copyright information in PMC

References

    1. Abouloifa H, Rokni Y, Bellaouchi R, Ghabbour N, Karboune S, Brasca M, Salah RB, Chihib NE, Saalaoui E, Asehraou A. Characterization of probiotic properties of antifungal Lactobacillus strains isolated from traditional fermenting green olives. Probiotics Antimicrob Proteins. 2020 Jun;12(2):683–696. https://doi.org/10.1007/s12602-019-09543-8. - PubMed
    1. Adesulu-Dahunsi AT, Sanni AI, Jeyaram K. Production, characterization and in vitro antioxidant activities of exopolysaccharide from Weissella cibaria GA44. LWT-Food Sci Technol. 2018 Jan;87:432–442. https://doi.org/10.1016/j.lwt.2017.09.013.
    1. Bacon RT, Ransom JR, Sofos JN, Kendall PA, Belk KE, Smith GC. Thermal inactivation of susceptible and multiantimicrobial-resistant Salmonella strains grown in the absence or presence of glucose. Appl Environ Microbiol. 2003 Jul;69(7):4123–4128. https://doi.org/10.1128/AEM.69.7.4123-4128.2003. - PMC - PubMed
    1. Bajaj BK, Andrabi T, Claes IJJ, Lebeer S. Bioprospecting for functionally-proficient potential probiotics. Curr Nutr Food Sci. 2014 Dec 12;10(4):251–263. https://doi.org/10.2174/1573401311666141215212331.
    1. Bao QH, Liu WJ, Yu J, Wang WH, Qing MJ, Chen X, Wang F, Zhang JC, Zhang WY, Qiao JM. et al. Isolation and identification of cultivable lactic acid bacteria in traditional yak milk products of Gansu Province in China. J Gen Appl Microbiol. 2012 Nov 5;58:95–105. https://doi.org/10.2323/jgam.58.95. - PubMed

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