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. 2022:2476:43-62.
doi: 10.1007/978-1-0716-2221-6_5.

Atomic Force Microscopy of DNA and DNA-Protein Interactions

Philip J Haynes  1   2   3 , Kavit H S Main  1   4 , Bernice Akpinar  1   2 , Alice L B Pyne  5   6
Affiliations

Atomic Force Microscopy of DNA and DNA-Protein Interactions

Philip J Haynes et al. Methods Mol Biol. 2022.

Abstract

Atomic force microscopy (AFM) is a microscopy technique that uses a sharp probe to trace a sample surface at nanometer resolution. For biological applications, one of its key advantages is its ability to visualize the substructure of single molecules and molecular complexes in an aqueous environment. Here, we describe the application of AFM to determine the secondary and tertiary structure of surface-bound DNA, and its interactions with proteins.

Keywords: AFM; Atomic force microscopy; DNA; DNA-protein binding; Double helix; Supercoiling.

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References

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