Contributions of MOF-808 to methane production from anaerobic digestion of waste activated sludge

Abstract

The bioconversion of waste activated sludge (WAS) into methane is usually limited by the poor hydrolysis of sludge and/or poor syntrophic methanogenesis during anaerobic digestion (AD). In this study, the underlying mechanism of MOF-808 enhancing hydrolysis and syntrophic methanogenesis during AD process of WAS was investigated. Experimentally, with the effects of MOF-808 (150 mg MOF-808/g Volatile Solid (VS)), the methane production and the proportion of methane in biogas increased by approximately 26.7% and 15.6%, respectively, and the lag phase of methanogenesis decreased by 50.8%, which indicate that MOF-808 enhanced the generation efficiency of methane. The changes in activities of main hydrolytic enzymes with and without MOF-808 (150 mg MOF-808/g VS) during AD process revealed that MOF-808 improved the enzymatic hydrolysis of sludge, and the abiotic hydrolysis of sludge extracellular organic substances by MOF-808 shows that the maximum proportion and the initial increasing rate of low-molecular weight fractions increased by 60% and 583.7%, respectively, indicating that MOF-808 can greatly enhance the hydrolysis degree and rate of sludge via abiotic effect. These demonstrate that MOF-808 enhanced both biological and abiotic hydrolysis of sludge during AD. In addition, changes in the concentrations of acetate kinase and volatile fatty acids (VFAs) with and without MOF-808 (150 mg MOF-808/g VS) during AD process showed that MOF-808 accelerated the bioconversion of VFAs to methane, suggesting MOF-808 has a positive effect on syntrophic metabolism for methanogenesis. Moreover, further analyses of the microbial community structure of sludge samples with and without MOF-808 (150 mg MOF-808/g VS) showed that MOF-808 enriched hydrogen-producing bacteria and mixotrophic methanogens (i.e. Methanosarcina), and changed the methanogenic pathway via accelerating proton transfer between syntrophic anaerobes, especially improving the reduction of CO₂ to methane, and resulting in highly efficient syntrophic methanogenesis. These findings, however, may provide an important reference for enhancing AD efficiency of WAS based on MOF-like materials.

Keywords: Anaerobic digestion; Biodegradation; Metal-organic frameworks; Methane production; Proton transfer; Sewage sludge.