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Comment
. 2022 Sep;85(3):334-363.
doi: 10.1016/j.jinf.2022.05.026. Epub 2022 May 27.

SARS-CoV-2 humoral and cellular immune responses in COVID-19 convalescent individuals with HIV

Denise Giannone  1 , María Belén Vecchione  1 , Alejandro Czernikier  1 , María Laura Polo  1 , Virginia Gonzalez Polo  1 , Leonel Cruces  2 , Yanina Ghiglione  1 , Silvia Balinotti  3 ; BBEI working group; Yesica Longueira  1 , Gabriela Turk  4 , Natalia Laufer  4 , Maria Florencia Quiroga  5
Affiliations
Comment

SARS-CoV-2 humoral and cellular immune responses in COVID-19 convalescent individuals with HIV

Denise Giannone et al. J Infect. 2022 Sep.
No abstract available

Keywords: Antibody responses; COVID-19,SARS-CoV-2; Cellular responses; HIV; HIV/SARS-CoV-2 coinfection; Humoral immunity.

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Conflict of interest statement

Declaration of Competing Interest The authors declare that they have no competing interests.

Figures

Figure 1
Figure 1
Clinical manifestations, anti-SARS-CoV-2 antibody responses, B and T cell phenotype in PWH and HIVneg individuals. (A) Frequency of occurrence of the depicted symptoms in PWH (red) and HIVneg (blue) cohorts. (B) IgG NOD values, (C) IgG titers, and (D) neutralizing Anti-SARS-CoV-2 antibodies were determined in plasma from PWH and HIVneg individuals (COVIDAR kit, Laboratorio Lemos S.R.L., Argentina). Normalized optical density (NOD) values were calculated by subtracting the cut-off value to each donor sample OD value, and the resulting value was divided by the mean positive control OD value. Mann-Whitney test was used. p < 0.05 were considered significant. Data are expressed as median and interquartile range. (E) Heatmap depicting from red (+1) to blue (-1) Spearman Rank correlation values between each parameter. p values per correlation are shown in those boxes where statistics were significant. **p < 0.01; ****p < 0.0001. Frequency of (F) antibody-secreting cells (ASC), (G) Tfh, and (H) CXCR3+ Tfh cells in SARS-CoV-2 convalescent PWH (n =25) and HIV-negative (n =24) individuals via traditional gating flow cytometric analysis. Each dot represents an individual donor. Data are expressed as median and interquartile range. (I) Spearman test (two-tailed) showing a negative correlation between IgG titters and frequency of Tfh in PWH.
Figure 2
Figure 2
Assessment of serum cytokines and chemokines and cellular immunity in PWH and HIVneg donors. (A) The concentrations of CCL8/IL8; CXCL10/IP10; IFN-γ; TNF-α; IL-17A; IL-10 and IL-6 were determined by a multiplex assay and flow cytometry. Data are depicted as the log-transformed concentration values (pg/mL). Each point represents an individual donor. Data are expressed as median and interquartile range. Significance was determined by two-tailed Mann−Whitney U test, **p < 0.01, ***p < 0.001, ****p < 0.0001. (B) IFN-γ ELISpot assays were performed to determine the frequency of Ag-experienced T cells in peripheral blood from the individuals enrolled. Stimulation of PBMCs with Spike (S) protein, RBD protein or Nucleocapside (N) protein was performed. Afterwards, IFN-γ producing cells were determinined as illustrated in the Supplementary Materials section. In order to compare group differences, data were normalized to media levels. Each dot represents an individual donor. Data are expressed as median and interquartile range. Significance was determined by two-tailed Mann−Whitney U test, *p < 0.05; **p < 0.01. A.U.: arbitrary units.
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References

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