SARS-CoV-2 Molecular Diagnostics in China

doi:10.1016/j.cll.2022.03.003

Review

. 2022 Jun;42(2):193-201.

doi: 10.1016/j.cll.2022.03.003. Epub 2022 Mar 4.

SARS-CoV-2 Molecular Diagnostics in China

Yanjun Lu¹, Ziyong Sun²

Affiliations

¹ Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
² Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. Electronic address: zysun@tjh.tjmu.edu.cn.

PMID: 35636821
PMCID: PMC8894802
DOI: 10.1016/j.cll.2022.03.003

Review

SARS-CoV-2 Molecular Diagnostics in China

Yanjun Lu et al. Clin Lab Med. 2022 Jun.

. 2022 Jun;42(2):193-201.

doi: 10.1016/j.cll.2022.03.003. Epub 2022 Mar 4.

Authors

Yanjun Lu¹, Ziyong Sun²

Affiliations

¹ Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
² Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. Electronic address: zysun@tjh.tjmu.edu.cn.

PMID: 35636821
PMCID: PMC8894802
DOI: 10.1016/j.cll.2022.03.003

Abstract

The COVID-19 pandemic remains a significant problem involving health systems worldwide. Several diagnostic methods are reported for detecting the coronavirus in clinical, research, and public health laboratories. rRT-PCR is considered the gold standard; however, as it required skilled personnel and special equipment, rapid antigen tests have been developed and used as first-line screening. The serologic testing of antibodies can also be used to enhance the detection sensitivity and accuracy, which are used to assess the overall infection rate. This review summarizes the molecular techniques and serologic assays widely used in China and discusses the advantages and disadvantages of these techniques.

Keywords: Molecular diagnosis; Real-time PCR; SARS-CoV-2; Serologic assays.

PubMed Disclaimer

Cited by

SARS-CoV-2, long COVID, prion disease and neurodegeneration.
Zhao Y, Jaber VR, Lukiw WJ. Zhao Y, et al. Front Neurosci. 2022 Sep 27;16:1002770. doi: 10.3389/fnins.2022.1002770. eCollection 2022. Front Neurosci. 2022. PMID: 36238082 Free PMC article. No abstract available.

References

1. Zhu N., Zhang D., Wang W., et al. A novel coronavirus from patients with pneumonia in China, 2019. N Engl J Med. 2020;382(8):727–733. - PMC - PubMed
1. Wang C., Horby P.W., Hayden F.G., et al. A novel coronavirus outbreak of global health concern. Lancet. 2020;395(10223):470–473. - PMC - PubMed
1. World Health Organization Coronavirus disease (COVID-19) outbreak situation. http://www.who.int/emergen cies/diseases/novel-coronavirus-2019 Available at. Accessed Feb 2022.
1. Li Z., Chen Q., Feng L., et al. Active case finding with case management: the key to tackling the COVID-19 pandemic. Lancet. 2020;396(10243):63–70. - PMC - PubMed
1. Chan J.F., Kok K.H., Zhu Z., et al. Genomic characterization of the 2019 novel human-pathogenic coronavirus isolated from a patient with atypical pneumonia after visiting Wuhan. Emerg Microbes Infect. 2020;9(1):221–236. - PMC - PubMed

Publication types

Actions

MeSH terms

Actions
Actions
Actions
Actions
Actions
Actions
Actions

LinkOut - more resources

Full Text Sources
Medical
- MedlinePlus Health Information
Miscellaneous
- NCI CPTAC Assay Portal

[1] Zhu N., Zhang D., Wang W., et al. A novel coronavirus from patients with pneumonia in China, 2019. N Engl J Med. 2020;382(8):727–733. - PMC - PubMed

[2] Zhu N., Zhang D., Wang W., et al. A novel coronavirus from patients with pneumonia in China, 2019. N Engl J Med. 2020;382(8):727–733. - PMC - PubMed

[3] Wang C., Horby P.W., Hayden F.G., et al. A novel coronavirus outbreak of global health concern. Lancet. 2020;395(10223):470–473. - PMC - PubMed

[4] Wang C., Horby P.W., Hayden F.G., et al. A novel coronavirus outbreak of global health concern. Lancet. 2020;395(10223):470–473. - PMC - PubMed

[5] World Health Organization Coronavirus disease (COVID-19) outbreak situation. http://www.who.int/emergen cies/diseases/novel-coronavirus-2019 Available at. Accessed Feb 2022.

[6] World Health Organization Coronavirus disease (COVID-19) outbreak situation. http://www.who.int/emergen cies/diseases/novel-coronavirus-2019 Available at. Accessed Feb 2022.

[7] Li Z., Chen Q., Feng L., et al. Active case finding with case management: the key to tackling the COVID-19 pandemic. Lancet. 2020;396(10243):63–70. - PMC - PubMed

[8] Li Z., Chen Q., Feng L., et al. Active case finding with case management: the key to tackling the COVID-19 pandemic. Lancet. 2020;396(10243):63–70. - PMC - PubMed

[9] Chan J.F., Kok K.H., Zhu Z., et al. Genomic characterization of the 2019 novel human-pathogenic coronavirus isolated from a patient with atypical pneumonia after visiting Wuhan. Emerg Microbes Infect. 2020;9(1):221–236. - PMC - PubMed

[10] Chan J.F., Kok K.H., Zhu Z., et al. Genomic characterization of the 2019 novel human-pathogenic coronavirus isolated from a patient with atypical pneumonia after visiting Wuhan. Emerg Microbes Infect. 2020;9(1):221–236. - PMC - PubMed

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

SARS-CoV-2 Molecular Diagnostics in China

Affiliations

SARS-CoV-2 Molecular Diagnostics in China

Authors

Affiliations

Abstract

Similar articles

Cited by

References

Publication types

MeSH terms

LinkOut - more resources

Full Text Sources

Medical

Miscellaneous