Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2022 Jun;42(2):193-201.
doi: 10.1016/j.cll.2022.03.003. Epub 2022 Mar 4.

SARS-CoV-2 Molecular Diagnostics in China

Yanjun Lu  1 , Ziyong Sun  2
Affiliations
Review

SARS-CoV-2 Molecular Diagnostics in China

Yanjun Lu et al. Clin Lab Med. 2022 Jun.

Abstract

The COVID-19 pandemic remains a significant problem involving health systems worldwide. Several diagnostic methods are reported for detecting the coronavirus in clinical, research, and public health laboratories. rRT-PCR is considered the gold standard; however, as it required skilled personnel and special equipment, rapid antigen tests have been developed and used as first-line screening. The serologic testing of antibodies can also be used to enhance the detection sensitivity and accuracy, which are used to assess the overall infection rate. This review summarizes the molecular techniques and serologic assays widely used in China and discusses the advantages and disadvantages of these techniques.

Keywords: Molecular diagnosis; Real-time PCR; SARS-CoV-2; Serologic assays.

PubMed Disclaimer

References

    1. Zhu N., Zhang D., Wang W., et al. A novel coronavirus from patients with pneumonia in China, 2019. N Engl J Med. 2020;382(8):727–733. - PMC - PubMed
    1. Wang C., Horby P.W., Hayden F.G., et al. A novel coronavirus outbreak of global health concern. Lancet. 2020;395(10223):470–473. - PMC - PubMed
    1. World Health Organization Coronavirus disease (COVID-19) outbreak situation. http://www.who.int/emergen cies/diseases/novel-coronavirus-2019 Available at. Accessed Feb 2022.
    1. Li Z., Chen Q., Feng L., et al. Active case finding with case management: the key to tackling the COVID-19 pandemic. Lancet. 2020;396(10243):63–70. - PMC - PubMed
    1. Chan J.F., Kok K.H., Zhu Z., et al. Genomic characterization of the 2019 novel human-pathogenic coronavirus isolated from a patient with atypical pneumonia after visiting Wuhan. Emerg Microbes Infect. 2020;9(1):221–236. - PMC - PubMed