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Review
. 2022 May 12:10:879476.
doi: 10.3389/fbioe.2022.879476. eCollection 2022.

Microalgal Biomass as Feedstock for Bacterial Production of PHA: Advances and Future Prospects

Florence Hui Ping Tan  1 , Najiah Nadir  2 , Kumar Sudesh  1
Affiliations
Review

Microalgal Biomass as Feedstock for Bacterial Production of PHA: Advances and Future Prospects

Florence Hui Ping Tan et al. Front Bioeng Biotechnol. .

Abstract

The search for biodegradable plastics has become the focus in combating the global plastic pollution crisis. Polyhydroxyalkanoates (PHAs) are renewable substitutes to petroleum-based plastics with the ability to completely mineralize in soil, compost, and marine environments. The preferred choice of PHA synthesis is from bacteria or archaea. However, microbial production of PHAs faces a major drawback due to high production costs attributed to the high price of organic substrates as compared to synthetic plastics. As such, microalgal biomass presents a low-cost solution as feedstock for PHA synthesis. Photoautotrophic microalgae are ubiquitous in our ecosystem and thrive from utilizing easily accessible light, carbon dioxide and inorganic nutrients. Biomass production from microalgae offers advantages that include high yields, effective carbon dioxide capture, efficient treatment of effluents and the usage of infertile land. Nevertheless, the success of large-scale PHA synthesis using microalgal biomass faces constraints that encompass the entire flow of the microalgal biomass production, i.e., from molecular aspects of the microalgae to cultivation conditions to harvesting and drying microalgal biomass along with the conversion of the biomass into PHA. This review discusses approaches such as optimization of growth conditions, improvement of the microalgal biomass manufacturing technologies as well as the genetic engineering of both microalgae and PHA-producing bacteria with the purpose of refining PHA production from microalgal biomass.

Keywords: biomass; microalgae; microalgal biomass production; microbial PHA synthesis; photoautotrophy; polyhydroxyalkanoates (PHA).

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Conflict of interest statement

Author NN was employed by company PETRONAS Research Sdn. Bhd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
Morphology of some microalgae commonly used for biomass generation viewed under light microscope. Spirulina (Arthrospira platensis) UMACC 161 (A) total magnification of ×400 and (A′) total magnification of ×1000; Chlamydomonas reinhardtii (B) total magnification of ×400 and (B′) total magnification of ×1000; Synechocystis sp. strain PCC6803 (C) total magnification of ×400 and (C′) total magnification of ×1000.
FIGURE 2
FIGURE 2
A model biorefinery process chain focusing on reducing energy requirement, economical cost. and maximizing biomass constituents.
See this image and copyright information in PMC

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