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. 2022 Mar 17:10:54-67.
doi: 10.1016/j.aninu.2022.01.009. eCollection 2022 Sep.

Non-starch polysaccharide-degrading enzymes may improve performance when included in wheat- but not maize-based diets fed to broiler chickens under subclinical necrotic enteritis challenge

Eunjoo Kim  1 , Amy F Moss  1 , Natalie K Morgan  1 , Kosar Gharib-Naseri  1 , Peter Ader  2 , Mingan Choct  1
Affiliations

Non-starch polysaccharide-degrading enzymes may improve performance when included in wheat- but not maize-based diets fed to broiler chickens under subclinical necrotic enteritis challenge

Eunjoo Kim et al. Anim Nutr. .

Abstract

The present study investigated whether supplementing fibre-degrading enzymes can ameliorate the severity of subclinical necrotic enteritis (NE) in broiler chickens offered wheat- or maize-based diets. A total of 1,544 mixed-sex broiler chickens were assigned to 16 experimental treatments as a 2 × 2 × 4 factorial arrangement of treatments. The factors were the following: NE challenge, yes or no; diet type, wheat- or maize-based; and enzyme supplementation, control (no enzyme), family 10 xylanase (XYN10), family 11 xylanase (XYN11) or β-mannanase (MAN). Each treatment was replicated 6 times, with 16 birds per replicate pen. A three-way challenge × diet type × enzyme interaction occurred for body weight at 21 d of age (P = 0.025) and overall feed conversion ratio (P = 0.001). In the non-challenged birds fed the wheat-based diet, supplementing MAN increased d 21 body weight compared to the control. In challenged birds fed the maize-based diet, supplemental XYN11 impeded body weight and overall FCR compared to the control. Birds offered the maize-based diet presented heavier relative gizzard weights at both 16 and 21 d of age (P < 0.001) and reduced liveability (P = 0.046) compared to those fed the wheat-based diet. Enzyme supplementation reduced ileal and jejunal digesta viscosity at 16 d of age only in birds fed the wheat-based diet (P < 0.001). XYN11 increased ileal digesta viscosity in birds fed the maize-based diet, and MAN reduced it in birds fed the wheat-based diet at 21 d of age (P = 0.030). Supplementing XYN11 improved ileal soluble non-starch polysaccharides (NSP) digestibility in birds fed the wheat-based diet compared to non-supplemented birds (P < 0.001). Birds fed the wheat-based diet displayed a higher abundance of Bifidobacterium, Lactobacillus and Enterobacteriaceae and butyric acid in the caeca at 16 d of age compared to birds fed the maize-based diet (P < 0.05). In conclusion, supplemental XYN11 exacerbated the negative impact of NE on growth performance in birds fed the maize-based diet. Supplementing wheat-based diets with fibre-degrading enzymes ameliorates production losses induced by NE.

Keywords: Broiler chicken; Carbohydrase; Mannanase; Necrotic enteritis; Prebiotic oligosaccharides; Xylanase.

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Conflict of interest statement

We declare that we have no financial and personal relationships with other people or organizations that can inappropriately influence our work, and there is no professional or other personal interest of any nature or kind in any product, service and/or company that could be construed as influencing the content of this paper.

References

    1. Abdollahi M., Ravindran V., Wester T., Ravindran G., Thomas D. Influence of conditioning temperature on performance, apparent metabolisable energy, ileal digestibility of starch and nitrogen and the quality of pellets, in broiler starters fed maize-and sorghum-based diets. Anim Feed Sci Technol. 2010;162:106–115.
    1. Annett C., Viste J., Chirino-Trejo M., Classen H., Middleton D., Simko E. Necrotic enteritis: effect of barley, wheat and corn diets on proliferation of Clostridium perfringens type A. Avian Pathol. 2002;31:598–601. - PubMed
    1. Aoac . 19th ed. 2012. Official methods of analysis of AOAC International.
    1. Bach Knudsen K.E., Jensen B.B., Andersen J., Hansen I. Gastrointestinal implications in pigs of wheat and oat fractions: 2. Microbial activity in the gastrointestinal tract. Br J Nutr. 1991;65:233–248. - PubMed
    1. Bao Y., Choct M. Dietary NSP nutrition and intestinal immune system for broiler chickens. World’s Poult Sci J. 2010;66:511–518.

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