Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Dec 15;29(4):738-750.
doi: 10.38212/2224-6614.3386.

Variations in bovine milk oligosaccharides after calving using p-aminobenzoic ethyl ester closed-ring labeling and negative ion electrospray LC/MS/MS

Han-Ju Chien  1 , Yi-Chun Ma  2 , Yi-Feng Zheng  1 , Cheng-Yu Kuo  1 , Wei-Chen Wang  1 , Guor-Rong Her  2 , Chien-Chen Lai  1   3   4   5   6
Affiliations

Variations in bovine milk oligosaccharides after calving using p-aminobenzoic ethyl ester closed-ring labeling and negative ion electrospray LC/MS/MS

Han-Ju Chien et al. J Food Drug Anal. .

Abstract

A strategy was proposed to analyze bovine milk oligosaccharides using p-aminobenzoic ethyl ester (ABEE) closed-ring labeling and C18 capillary liquid chromatography negative ion electrospray tandem mass spectrometry. Linkage specific fragment ions were used to identify oligosaccharide isomers. By constructing the mass chromatograms using linkage specific fragment ions, isomers were differentiated based on m/z values as well as temporal separation provided by liquid chromatography. In addition to disialyllactose and the single isomer lacto-N-neohexaose, four pairs of linkage isomers including 3'/6'-sialyllactose (3'/6'-SL), 3'/6'-sialyllactosamine (3'/6'-SLN), 3'/6'-sialylgalactosyl-lactose (3'/6'-SGL), and lacto-N-tetraose/lacto-N-neotetraose (LNT/LNnT) in bovine milk were investigated. Variations of bovine milk oligosaccharides in a lactation period of 72 h after calving were studied. Sialylated oligosaccharide was found to be distinctively more abundant in milk of the first 24 h, decreasing in successive milkings. For the first time, the variation of lacto-N-tetraose in bovine milk was reported.

PubMed Disclaimer

Conflict of interest statement

Conflicts of interest

The authors declare that they have no conflict of interest.

Figures

Fig. 1
Fig. 1
Negative ion ESI-MS1 and MS2 mass chromatograms of ABEE-labeled SL and SLN standards.
Fig. 2
Fig. 2
Negative ion ESI-MS2 spectra of (A) the early eluting isomer (6SGL) and (B) the late eluting isomer (3SGL) in bovine milk.
Fig. 3
Fig. 3
Mass chromatograms of oligosaccharides in bovine milk.
Fig. 4
Fig. 4
Variation of oligosaccharides after 24, 48, and 72 h of lactation.
See this image and copyright information in PMC

References

    1. Kunz C, Rudloff S, Baier W, Klein N, Strobel S. Oligosaccharides in human milk: structural, functional, and metabolic aspects. Annu Rev Nutr. 2000;20:699–722. - PubMed
    1. Newburg DS, Ruiz-Palacios GM, Morrow AL. Human milk glycans protect infants against enteric pathogens. Annu Rev Nutr. 2005;25:37–58. - PubMed
    1. Boehm G, Stahl B. Oligosaccharides from milk. J Nutr. 2007;137:847S. 9S. - PubMed
    1. Tao N, DePeters E, Freeman S, German J, Grimm R, Lebrilla CB. Bovine milk glycome. J Dairy Res. 2008;91:3768–78. - PubMed
    1. McJarrow P, van Amelsfort-Schoonbeek J. Bovine sialyl oligosaccharides: seasonal variations in their concentrations in milk, and a comparison of the colostrums of Jersey and Friesian cows. Int Dairy J. 2004;14:571–9.

Publication types

LinkOut - more resources