Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1986;27(3):410-20.
doi: 10.1186/BF03548155.

Counter current line absorption immunoelectrophoresis in an alternative diagnostic screening test to counter current immunoelectrophoresis in Aleutian disease (AD) eradication programs

B Aasted, S Alexandersen, A Cohn, M Hansen
Comparative Study

Counter current line absorption immunoelectrophoresis in an alternative diagnostic screening test to counter current immunoelectrophoresis in Aleutian disease (AD) eradication programs

B Aasted et al. Acta Vet Scand. 1986.

Abstract
in English, German

Counter current immunoelectrophoresis (CCIE) is the diagnostic method used in the ongoing Aleutian disease virus eradication program on Danish mink farms. There has been an increasing demand for an alternative diagnostic test especially to evaluate suspected false positive CCIE reactions. We compared test results of a number of negative and positive mink sera in indirect counter current immunoelectrophoresis (ICCIE), counter current line absorption immunoelectrophoresis (CCLAIE) and radio immuno assay (RIA) with test results from counter current immunoelectrophoresis and found that counter current line absorption immunoelectrophoresis is the best alternative diagnostic screening test to counter current immunoelectrophoresis for Aleutian disease eradication programs. Not only proved the CCLAIE test to be useful for evaluation of doubtfully positive CCIE reactions, but it was found to have a higher sensitivity than the CCIE test.

Modstrømselektroforese er den diagnostiske metode, der i dag brages i mink plasmacytose udryddelsesprogrammet for danske minkfarmen. Det har vist sig, at der er et stigende behov for en alternativ diagnostisk metode specielt til at benytte ved evaluering af mulige falske positive reaktioner i modstrømselektroforese. Vi sammenlignede analyseresultater fra 3321 negative og positive mink sera i den indirekte modstrømselektroforese, modstrøms linie absorption immunelektroforese og radio immun assay med analyseresultater fra modstrøms elektroforese og fandt, at modstrøms linie absorption immunelektroforese er det bedste alternativ til modstrømselektroforese i mink plasmacytose udryddelsesprogrammet. Modstrøms linie absorption immun-elektroforese viste sig ikke blot at være nyttig til evaluering af mulige mistænkelige positive reaktioner i modstrømselektroforese, men den viste sig også at have en større følsomhed end modstrømselektroforese.

PubMed Disclaimer

References

    1. Aasted B, Cohn A. Inhibition of precipitation in counter current electrophoresis. A sensitive method for detection of mink antibodies to Aleutian disease virus. Acta path, microbiol. immunol. scand. Sect. G. 1982;90:15–19. - PubMed
    1. Aasted B, Bloom ME. Mink with Aleutian disease have high- affinity antiviral antibodies. Scand. J. Immunol. 1984;19:411–418. doi: 10.1111/j.1365-3083.1984.tb00949.x. - DOI - PubMed
    1. Immunol. scand. Sect. G. 1985.
    1. Alexandersen S, Hau J, Aasted B, Poulsen OM. Thin-layer counter current line absorption immunoelectrophoretic analysis of antigens and antibodies to Aleutian disease virus — a mink parvovirus. Electrophoresis. 1985;6:535–538. doi: 10.1002/elps.1150061104. - DOI
    1. Cho HJ, Ingram DG. Antigen and antibody in Aleutian disease in mink. I. Precipitation reaction by agar-gel electrophoresis. J. Immunol. 1972;108:555–557. - PubMed

Publication types

LinkOut - more resources