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. 2022 Feb;25(2):254-262.
doi: 10.22038/IJBMS.2022.57780.12853.

The effects of combined resveratrol and high intensity interval training on the hippocampus in aged male rats: An investigation into some signaling pathways related to mitochondria

Maryam Amirazodi  1   2 , Amin Mehrabi  1   3 , Mohammad Amin Rajizadeh  1   4 , Mohammad Abbas Bejeshk  4 , Khadijeh Esmaeilpour  1 , Farhad Daryanoosh  5 , Abbasali Gaeini  6
Affiliations

The effects of combined resveratrol and high intensity interval training on the hippocampus in aged male rats: An investigation into some signaling pathways related to mitochondria

Maryam Amirazodi et al. Iran J Basic Med Sci. 2022 Feb.

Abstract

Objectives: High-intensity interval training (HIIT) is a shape of interval training that provides ameliorated athletic capacity and has a good effect on health. Resveratrol is a natural polyphenol abundant in grapes and red wine and has been demonstrated to apply various useful health impacts on the body. This research aimed to evaluate the interactive effects of swimming HIIT and resveratrol consumption on SIRTs 3 & 4, NAD+/NADH, AMPK and SOD2 expression in aged rats.

Materials and methods: In total, forty-five old male albino rats (Wistar) with the age of twenty months were allocated into 5 groups randomly. Control group (Ctrl), Swimming HIIT group (Ex: Exercise), Swimming HIIT with Resveratrol consumption group (R+Ex), Resveratrol consumption group (R) and solvent of resveratrol consumption group (vehicle). R+Ex group accomplished the exercise and consumed resveratrol (10 mg/kg/day, gavage) for 6 weeks.

Results: HIIT & resveratrol significantly increased NAD+/NADH, SOD 2 and AMPK in the aged rats. HIIT increased SIRT3, but resveratrol reduced it. As for SIRT4, HIIT decreased it, while resveratrol positively affected it.

Conclusion: Resveratrol and HIIT, especially their combination, have anti-oxidant and anti-aging effects on the hippocampus of old rats.

Keywords: Aging; HIIT exercise; Hippocampus; Oxidative stress; Rat; Resveratrol; Sirtuins.

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Conflict of interest statement

The authors declare there are no conflicts of interest.

Figures

Figure 1
Figure 1
The effect of HIIT and Resveratrol on SIRT 3 expression in hippocampus of aged rats. There was a significant increase in SIRT 3 expression in Ex and R+Ex groups and a reduced expression in R group compared to Ctrl group. Mean±SD , (**) P<0.01 & (***) P<0.001
Figure 2
Figure 2
The effect of HIIT and Resveratrol on SIRT 4 expression in hippocampus of aged rats. There was a significant increase in SIRT 4 expression in R and R+Ex groups and a reduced expression in Ex group compared to Ctrl group. Mean±SD , (***) P<0.001
Figure 3
Figure 3
The effect of HIIT and Resveratrol on SOD 2 expression in hippocampus of aged rats. There was a significant increase in SOD 2 expression in R and R+Ex and Ex groups compared to Ctrl group. Mean±SD , (*) P<0.05 , (***) P<0.001
Figure 4
Figure 4
The effect of HIIT and Resveratrol on AMPK expression in hippocampus of aged rats. There was a significant increase in AMPK expression in R and R+Ex and Ex groups compared to Ctrl group. Mean±SD , (*) P<0.05 , (**) P<0.01
Figure 5
Figure 5
The effect of HIIT and Resveratrol on NAD+/NADH in hippocampus of aged rats. There was a significant increase in NAD+/NADH in R and R+Ex and Ex groups compared to Ctrl group. Mean±SD, (**) P<0.01 , (***) P<0.001
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References

    1. Finch CE, Hayflick L. Handbook of the biology of aging: Van Nostrand Reinhold Co. 1977.
    1. Harman D. Aging: overview. Annals of the New York Academy of Sciences. 2001;928 - PubMed
    1. Kohn RR. Aging and age-related diseases normal processes. Aging (New York) 1985;28
    1. Harman D. Aging: prospects for further increases in the functional life span. Age. 1994;17:119–146.
    1. Lowell BB, Shulman GI. Mitochondrial dysfunction and type 2 diabetes. Science. 2005;307:384–387. - PubMed

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