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. 2022 Jul-Aug;12(4):454-457.
doi: 10.1016/j.jobcr.2022.05.015. Epub 2022 May 28.

Depigmenting effect of banana stem and flower on melanocytes

Sowmya N K  1 , Goriparthi Neeharika Sree  1 , Pooja Patil  1 , D S Mehta  1
Affiliations

Depigmenting effect of banana stem and flower on melanocytes

Sowmya N K et al. J Oral Biol Craniofac Res. 2022 Jul-Aug.

Erratum in

Abstract

Gingival repigmentation is an inevitable hindrance among different procedures accepted for gingival depigmentation. To overcome this, there is a need for the procedure that can delay the duration of reappearance of pigmentation. A number of studies using herbal extracts with antioxidant property shown to have anti melanogenic effect. In the present in - vitro study, we investigated the effect of banana stem and flower extracts on melanocytes, as Banana stem and flower are rich in polyphenols and flavonoids, which are potent antioxidants. The melanocytes were exposed to ethanolic extract of banana stem and flower at 2 concentrations (100 μgm, 150 μgm) for 72 h. The cellular melanin contents were measured using Bradford assay which depicted the reduction in the melanin content and Resazurin assay was used for assessment of cell viability showed no significant cytotoxic effect of banana stem and flower on the cells. The cells exposed to higher concentration (150 μgm) of banana stem and flower showed significant reduction in melanin content. Flower extract showed better reduction in the melanin content. Based on these results both banana flower and stem can be tried as potent gingival depigmenting agent.

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Figures

Fig. 1
Fig. 1
Comparison of Melanin absorbance at 492 nm for 100 μgm and 150 μgm of banana stem and flower extracts. Data are expressed as amount of melanin protein absorbed by spectrophotometry.
Fig. 2
Fig. 2
Comparison of the effect of ethanolic extract of banana stem and flower on melanocytes. Data are expressed as amount of melanin protein absorbed by spectrophotometry at 492 nm.
Fig. 3
Fig. 3
Bright field and fluorescent microscopic image melanocytes treated with control, stem and flower. Banana flower showed reduced green fluorescence illustrating decline in number of melanocytes.
Fig. 4
Fig. 4
Comparison of the effect of the banana stem and flower on cell viability using Resazurin Assay test. Data are expressed based on the spectrometric analysis with the excitation at 530 nm and emission at 580 nm.
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