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. 2022:2518:237-251.
doi: 10.1007/978-1-0716-2421-0_14.

Harnessing CRISPR-Cas9 for Epigenetic Engineering

Rosa S Guerra-Resendez  1 , Isaac B Hilton  2   3   4
Affiliations

Harnessing CRISPR-Cas9 for Epigenetic Engineering

Rosa S Guerra-Resendez et al. Methods Mol Biol. 2022.

Abstract

Epigenome editing has become more precise and effective by coupling epigenetic effectors to the dCas9 protein and targeting regulatory regions such as promoters and enhancers. Here, we describe a basic methodology for performing an epigenome editing experiment, starting from gRNA design and cloning to transiently transfecting the gRNA plasmid and the CRISPR/dCas9-based epigenetic effector and finalizing with chromatin immunoprecipitation (ChIP) to validate changes in epigenetic state at a targeted genomic region.

Keywords: CRISPR; Deactivated Cas9; Epigenome editing; Guide RNA; Synthetic biology.

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References

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