Media engineering in marine diatom Phaeodactylum tricornutum employing cost-effective substrates for sustainable production of high-value renewables
- PMID: 35666486
- DOI: 10.1002/biot.202100684
Media engineering in marine diatom Phaeodactylum tricornutum employing cost-effective substrates for sustainable production of high-value renewables
Abstract
Phaeodactylum tricornutum is a marine diatom, rich in omega-3 polyunsaturated fatty acids especially eicosapentaenoic acid (EPA) and brown pigment, that is, fucoxanthin. These high-value renewables (HVRs) have a high commercial and nutritional relevance. In this study, our focus was to enhance the productivities of such renewables by employing media engineering strategy via., photoautotrophic (P1, P2, P3) and mixotrophic (M1, M2, M3, M4) modes of cultivation with varying substrate combinations of carbon (glycerol: 0.1 m) and nitrogen (urea: 441 mm and/or sodium nitrate: 882 mm). Our results demonstrate that mixotrophic [M4] condition supplemented with glycerol (0.1 m) and urea (441 mm) feed enhanced productivities (mg L-1 day-1 ) as follows: biomass (770.0), total proteins (36.0), total lipids (22.0), total carbohydrates (23.0) with fatty acid methyl esters (9.6), EPA (2.7), and fucoxanthin (1.1), respectively. The overall yield of EPA represents 28% of total fatty acids in the mixotrophic [M4] condition. In conclusion, our improved strategy of feeding urea to a glycerol-supplemented medium defines a new efficient biomass valorization paradigm with cost-effective substrates for the production of HVRs in oleaginous diatoms P. tricornutum.
Keywords: Phaeodactylum tricornutum; eicosapentaenoic acid; fucoxanthin; high-value renewables; microalgae; mixotrophy; urea.
© 2022 Wiley-VCH GmbH.
References
REFERENCES
-
- Rehmanji, M., Singh, R., Nesamma, A. A., Khan, N. J., Fatma, T., & Narula, A. (2022). Multifaceted applications of microalgal biomass valorization to enriched biorenewables, a review of futuristic biorefinery paradigm. Bioresource Technology Reports, 17, 100972.
-
- Singh, R., Parihar, P., Singh, M., Bajguz, A., Kumar, J., & Singh, S. (2017). Uncovering potential applications of cyanobacteria and algal metabolites in biology, agriculture and medicine: Current status and future prospects. Frontiers in Microbiology, 8, 515.
-
- Branco-Vieira, M., Martin, S. S., Agurto, C., Santos, M. A., Freitas, M. A. V., & Caetano, N. S. (2017). Analyzing Phaeodactylum tricornutum lipid profile for biodiesel production. Energy Procedia, 136, 369-373.
-
- Hao, X., Luo, L., Jouhet, J., Rébeillé, F., Maréchal, E., & Hu, H. (2018). Enhanced triacylglycerol production in the diatom Phaeodactylum tricornutum by inactivation of a Hotdog-fold thioesterase gene using TALEN-based targeted mutagenesis. Biotechnology for Biofuels, 11, 312.
-
- Pudney, A., Gandini, C., Economou, C. K., Smith, R., Goddard, P., & Napier, J. A. (2019). Multifunctionalizing the marine diatom Phaeodactylum tricornutum for sustainable co-production of omega-3 long chain polyunsaturated fatty acids and recombinant phytase. Science Reports, 9, 11444.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials