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. 2022 May 27:2022:8243192.
doi: 10.1155/2022/8243192. eCollection 2022.

Dissemination and Genetic Relatedness of Multidrug-Resistant and Extensively Drug-Resistant Acinetobacter baumannii Isolates from a Burn Hospital in Iraq

Aras A K Shali  1 , Paywast J Jalal  1 , Sehand K Arif  1
Affiliations

Dissemination and Genetic Relatedness of Multidrug-Resistant and Extensively Drug-Resistant Acinetobacter baumannii Isolates from a Burn Hospital in Iraq

Aras A K Shali et al. Can J Infect Dis Med Microbiol. .

Abstract

Acinetobacter baumannii is an aggressive opportunistic bacterial pathogen that causes severe nosocomial infections, especially among burn patients. An increasing number of hospitals-acquired infections have been reported all over the world. However, little attention has been paid to the relatedness between A. baumannii isolates from different hospital environments and patients. In this study, 27 isolates were collected from the Burn and Plastic Surgery Hospital of Al Sulaymaniyah City, Iraq, from January through December 2019 (11 from patients and 16 from the wards environment), identified to species level as A. baumannii using Vitek 2 system and molecular detection of 16S rRNA gene, and then confirmed by targeting the bla OXA-51 gene. Moreover, the isolates were characterized by means of automated antimicrobial susceptibility assay, antimicrobial-resistant patterns, a phenotypic method using a combined disk test, and molecular methods for the detection of class A and C β-lactamase genes, and finally, the genetic relatedness was classified. Antimicrobial susceptibility testing showed that 63% (17/27) of the retrieved A. baumannii isolates were extensively drug-resistant to 8/9 antimicrobial classes. Furthermore, 37% (10/27) of the isolates were classified as multidrug-resistant; 8 isolates exhibited similar resistant patterns and the other two isolates showed 2 different patterns, while resistance was greater in isolates from patients than from the ward environment. Combined disk test showed that two isolates contained extended-spectrum β-lactamase. All isolates carried bla TEM-1, and two copies of the bla CTX-1 gene were indicated in one isolate, while bla SHV was absent in all isolates. Twenty-four isolates carried the bla AmpC gene; among them, 3 isolates harbored the insertion sequence ISAba-1 upstream to the gene. Using Enterobacterial Repetitive Intergenic Consensus PCR, the isolates were clustered into 6 distinct types; among them, two clusters, each of four strains, were classified to contain isolates from both patients and environments. The clusters of similar genotypes were found in inpatients as well as the environments of different wards during time periods, suggesting transmission within the hospital. Identification of possible infection sources and controlling the transmission of these aggressive resistance strains should be strictly conducted.

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Conflict of interest statement

The authors declare there are no conflicts of interest.

Figures

Figure 1
Figure 1
The heat map illustrating the different degrees of susceptibility of the retrieved A. baumannii to different antimicrobial agents used in this study.
Figure 2
Figure 2
Percentage of nonsusceptibility of A. baumannii to different antibiotic classes.
Figure 3
Figure 3
Combined disk test for phenotypic detection of ESBL-producing A. baumannii.
Figure 4
Figure 4
ERIC pattern of A. baumannii from patients' isolates.
Figure 5
Figure 5
ERIC pattern of A. baumannii from environment isolates.
Figure 6
Figure 6
Dendrogram of ERIC-PCR analysis for 27 A. baumannii. (a–d) Different clusters.
See this image and copyright information in PMC

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