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. 2022 Jun 23;58(51):7192-7195.
doi: 10.1039/d2cc00200k.

Charge detection mass spectrometry on human-amplified fibrils from different synucleinopathies

Aikaterini Tsirkou  1 , Flora Kaczorowski  2   3   4 , Mathieu Verdurand  2   3   4 , Rana Raffoul  1 , Jonathan Pansieri  5 , Isabelle Quadrio  2   3   4 , Fabien Chauveau  4 , Rodolphe Antoine  1
Affiliations

Charge detection mass spectrometry on human-amplified fibrils from different synucleinopathies

Aikaterini Tsirkou et al. Chem Commun (Camb). .

Abstract

Amyloid fibrils are self-assembled mesoscopic protein aggregates, which can accumulate to form deposits or plaques in the brain. In vitro amplification of fibrils can be achieved with real-time quaking-induced conversion (RT-QuIC). However, this emerging technique would benefit from a complementary method to assess structural properties of the amplification products. This work demonstrates the feasibility of nanospray-charge-detection-mass-spectrometry (CDMS) performed on α-synuclein (αSyn) fibrils amplified from human brains with Parkinson's disease (PD) or Dementia with Lewy bodies (DLB) and its synergistic combination with RT-QuIC.

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