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. 1978 Jul;14(1):88-94.
doi: 10.1128/AAC.14.1.88.

Fluorescent assay for estimating the binding of erythromycin derivatives to ribosomes

P Brandt-Rauf, R Vince, R LeMahieu, S Pestka

Fluorescent assay for estimating the binding of erythromycin derivatives to ribosomes

P Brandt-Rauf et al. Antimicrob Agents Chemother. 1978 Jul.

Abstract

A fluorescent erythromycin derivative was used to determine the binding of erythromycin derivatives to ribosomes. The assay is rapid, sensitive, and convenient. Because measurements are made in solution, they represent equilibrium conditions, unlike filter binding assays which perturb the equilibrium. Results correlate well with measurements made by other techniques.

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