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. 2021 Oct-Dec;62(4):1029-1034.
doi: 10.47162/RJME.62.4.15.

Role of ProEx C immunocytochemistry in cervical high-grade squamous intraepithelial lesions detection

Carmen Ungureanu  1 , Demetra Gabriela Socolov, Gabriela Anton, Eugenia Moroşan, Laura Mihaela Trandafir, Ludmila Lozneanu, Mioara Florentina Trandafirescu, Elena Cojocaru
Affiliations

Role of ProEx C immunocytochemistry in cervical high-grade squamous intraepithelial lesions detection

Carmen Ungureanu et al. Rom J Morphol Embryol. 2021 Oct-Dec.

Abstract

Background: Screening programs using Papanicolaou smear along with human papillomavirus (HPV) testing led to a significantly decrease of cervical cancer rates. Nevertheless, both assessments have limited specificity for revealing cervical high-grade lesions. The main problem is how to identify the real precursor of cervical squamous cell carcinomas (SCC), namely high-grade squamous intraepithelial lesions (HSIL).

Aim: The aim of our study was to conclude if ProEx C might be used as a marker for high-grade cervical intraepithelial neoplasia (CIN).

Materials and methods: In this study, we detected the immunochemical expression of anti-ProEx C antibody in liquid-based cytology (LBC) samples. We analyzed a total number of 125 cervical cytology specimens.

Results: In 48% of all cases, ProEx C was found to be positive. The percentage increased from 0% in negative for intraepithelial lesion or malignancy (NILM) cases to 100% in SCC cases.

Conclusions: ProEx C may be utilized to improve the accuracy of cytological diagnosis on cervical smears, according to the findings of this study. This marker is also useful in detecting unrevealed high-grade lesions on atypical squamous cells of undetermined significance (ASC-US), low-grade squamous intraepithelial lesions (LSIL) smears, being very useful in establishing the conduct of these cases.

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Conflict of interest statement

The authors declare that they have no conflict of interests.

Figures

Figure 1
Figure 1
ASC-US: nuclear marking for ProEx C (immunocytochemistry, ×400). ASC-US: Atypical squamous cells of undetermined significance
Figure 2
Figure 2
LSIL: nuclear labeling for ProEx C in dysplastic cells (immunocytochemistry, ×1000). Low-grade squamous intraepithelial lesion
Figure 3
Figure 3
HSIL: nuclear labeling for ProEx C in dysplastic cells (immunocytochemistry, ×400). HSIL: High-grade squamous intraepithelial lesion
Figure 4
Figure 4
SCC: nuclear labeling for ProEx C in dysplastic cells (immunocytochemistry, ×400). SCC: Squamous cell carcinoma
See this image and copyright information in PMC

References

    1. Kim G. Harald zur Hausen’s experiments on human papilloma-virus causing cervical cancer (1976–1987) Embryo Project Encyclopedia, Arizona State University, School of Life Sciences, Center for Biology and Society. 2017 http://embryo.asu.edu/handle/10776/11444
    1. Perkins RB, Guido RS, Castle PE, Chelmow D, Einstein MH, Garcia F, Huh WK, Kim JJ, Moscicki AB, Nayar R, Saraiya M, Sawaya GF, Wentzensen N, Schiffman M, 2019 ASCCP Risk-Based Management Consensus Guidelines Committee ASCCP risk-based management consensus guidelines for abnormal cervical cancer screening tests and cancer precursors. J Low Genit Tract Dis. 2020;24(2):102–131. - PMC - PubMed
    1. Khieu M, Butler SL. In: StatPearls, editor. Treasure Island FL USA: StatPearls Publishing; 2022. High grade squamous intraepithelial lesion (HSIL. - PubMed
    1. Raţiu AC, Secoşan CA, Balint O, Sas I, Grigoraş D, Ilina RŞ, Jianu AM, Motoc AGM, Pirtea LC. The importance of immunocytochemistry in the detection of high-grade cervical lesions. Rom J Morphol Embryol. 2017;58(4):1151–1156. - PubMed
    1. Buitrago-Pérez A, Garaulet G, Vázquez-Carballo A, Paramio JM, García-Escudero R. Molecular signature of HPV-induced carcinogenesis: pRb, p53 and gene expression profiling. Curr Genomics. 2009;10(1):26–34. - PMC - PubMed