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. 2022:2517:43-51.
doi: 10.1007/978-1-0716-2417-3_4.

qPCR Detection of Candida auris Using the GPS™ CanAur MONODOSE dtec-qPCR Test

Antonio Martínez-Murcia  1   2 , Gema Bru  3 , Aaron Navarro  3
Affiliations

qPCR Detection of Candida auris Using the GPS™ CanAur MONODOSE dtec-qPCR Test

Antonio Martínez-Murcia et al. Methods Mol Biol. 2022.

Abstract

Candida auris is a multidrug-resistant pathogenic ascomycete yeast of increasing health concern. C. auris colonizes patient's skin and can persist for weeks on surfaces, so it can be transmitted within and between hospitals. The most common diagnostic platforms in microbiology use reference databases that have not yet incorporated C. auris, misidentifying it. This chapter describes how to detect C. auris by qPCR with the GPS™ CanAur MONODOSE dtec-qPCR Test (Alicante, Spain) in less than 45 min, using ready-to-use tubes with all the components dehydrated. This commercial kit was subjected to validation following the guidelines of the UNE-EN ISO/IEC 17025:2005 and French Standard NF T90-471:2010.

Keywords: Candida auris diagnosis; DNA extraction; UNE–EN ISO/IEC 17025:2005; Validation; qPCR.

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