Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022:2517:129-153.
doi: 10.1007/978-1-0716-2417-3_10.

Cell Viability Assays for Candida auris

Jason L Brown  1   2 , Bryn Short  3   4 , Alicia Ware  4   5 , Leighann Sherry  3   4 , Ryan Kean  4   5 , Gordon Ramage  6   7
Affiliations

Cell Viability Assays for Candida auris

Jason L Brown et al. Methods Mol Biol. 2022.

Abstract

Cell viability assays are useful for assessing the efficacy of antifungal therapeutics and disinfection strategies in vitro. In recent years these assays have been fundamental for the testing of conventional and novel therapies against the nosocomial fungal pathogen Candida auris. Here we provide detailed descriptions of methods for assessing cellular viability of Candida auris in vitro, such as metabolic assays (XTT and resazurin), colony-forming unit counting, live/dead quantitative PCR, and fluorescent staining for microscopic analyses.

Keywords: Candida auris; Cell viability; Colony-forming units; Fluorescent staining; Live/dead qPCR; Resazurin; XTT.

PubMed Disclaimer

References

    1. Ramage G, Vande Walle K, Wickes BL, Lopez-Ribot JL (2001) Standardized method for in vitro antifungal susceptibility testing of Candida albicans biofilms. Antimicrob Agents Chemother 45(9):2475–2479 - DOI
    1. Pierce CG, Uppuluri P, Tristan AR, Wormley FL Jr, Mowat E, Ramage G, Lopez-Ribot JL (2008) A simple and reproducible 96-well plate-based method for the formation of fungal biofilms and its application to antifungal susceptibility testing. Nat Protoc 3(9):1494–1500 - DOI
    1. Pierce CG, Uppuluri P, Tummala S, Lopez-Ribot JL (2010) A 96 well microtiter plate-based method for monitoring formation and antifungal susceptibility testing of Candida albicans biofilms. J Vis Exp 44:2287
    1. Dalecki AG, Crawford CL, Wolschendorf F (2016) Targeting biofilm associated staphylococcus aureus using resazurin based drug-susceptibility assay. J Vis Exp 111:53925
    1. Van den Driessche F, Rigole P, Brackman G, Coenye T (2014) Optimization of resazurin-based viability staining for quantification of microbial biofilms. J Microbiol Methods 98:31–34 - DOI

Substances

LinkOut - more resources