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. 2022 Aug;115(8):995-1007.
doi: 10.1007/s10482-022-01750-8. Epub 2022 Jun 8.

Formicincola oecophyllae gen. nov. sp. nov., a novel member of the family Acetobacteraceae isolated from the weaver ant Oecophylla smaragdina

Kah-Ooi Chua  1   2 , Yvonne Jing Mei Liew  3   4 , Wah-Seng See-Too  2 , Jia-Yi Tan  2 , Hoi-Sen Yong  2 , Wai-Fong Yin  2 , Kok-Gan Chan  5   6
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Formicincola oecophyllae gen. nov. sp. nov., a novel member of the family Acetobacteraceae isolated from the weaver ant Oecophylla smaragdina

Kah-Ooi Chua et al. Antonie Van Leeuwenhoek. 2022 Aug.

Abstract

In this study, a novel bacterium designated F3b2T was isolated from the gut sample of weaver ant Oecophylla smaragdina and characterised. Strain F3b2T was a Gram-negative, aerobic, non-motile, ovoid-shaped bacterium and grows optimally at 28-30 °C. Its major respiratory quinone is ubiquinone 10 (Q-10) and the major fatty acids are C18:1 ω7c, C19:0 cyclo ω8c and C16:0, representing 85% of the total fatty acids. The 16S rRNA gene sequence of strain F3b2T was highest in similarity to that of Oecophyllibacter saccharovorans DSM106907T and Swingsia samuieinsis NBRC 107927T at 94.35% and 91.96%, respectively. A 16S rRNA gene-based phylogenetic analysis and a core genes-based phylogenomic analysis placed strain F3b2T in a distinct lineage in the family Acetobacteraceae. The phylogenetic placement was supported by lower than species delineation threshold average nucleotide identity (ANI) (≤ 70.2%), in silico DNA-DNA hybridization (DDH) (≤ 39.5%) and average amino acid identity (AAI) (≤ 63.5%) values between strain F3b2T and closest neighbours. These overall genome relatedness indices also supported the assignment of strain F3b2T to a novel genus within Acetobacteraceae. The genome of strain F3b2T was 1.96 Mb with 60.4% G + C DNA content. Based on these results, strain F3b2T represented a novel taxon of Acetobacteraceae, for which we proposed the name Formicincola oecophyllae gen. nov. sp. nov., and strain F3b2T (= LMG 30590T = DSM 106908T = NBRC 113640T = KCTC 62951T) as the type strain.

Keywords: Asian weaver ant; Insect; Polyphasic taxonomy; Rhodospirillales; Symbiotic bacterium.

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References

    1. Alonso DP, Mancini MV, Damiani C, Cappelli A, Ricci I, Alvarez MVN, Bandi C, Ribolla PEM, Favia G (2018) Genome reduction in the mosquito symbiont Asaia. Genome Biol Evol 11:1–10 - PMC - DOI
    1. Asai T, Iizuka H, Komagata K (1964) The flagellation and taxonomy of genera Gluconobacter and Acetobacter with reference to the existence of intermediate strains. J Gen Appl Microbiol 10:95–126 - DOI
    1. Aydin YA, Aksoy ND (2009) Isolation of cellulose producing bacteria from wastes of vinegar fermentation. In: WCECS 2009: world congress on engineering and computer science, vol. 1. International Association Engineers-Iaeng, Hong Kong, pp 20–22
    1. Blüthgen N, Fiedler K (2002) Interactions between weaver ants Oecophylla smaragdina, homopterans, trees and lianas in an Australian rain forest canopy. J Anim Ecol 71:793–801 - DOI
    1. Brown BP, Wernegreen JJ (2016) Deep divergence and rapid evolutionary rates in gut-associated Acetobacteraceae of ants. BMC Microbiol 16:140 - PubMed - PMC - DOI

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