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. 2022 Jun 23;386(25):2436-2438.
doi: 10.1056/NEJMc2202861. Epub 2022 Jun 8.

SARS-CoV-2 Evolution and Immune Escape in Immunocompromised Patients

Erin M Scherer  1 , Ahmed Babiker  1 , Max W Adelman  1 , Brent Allman  1 , Autum Key  1 , Jennifer M Kleinhenz  1 , Rose M Langsjoen  1 , Phuong-Vi Nguyen  1 , Ivy Onyechi  1 , Jacob D Sherman  1 , Trevor W Simon  1 , Hannah Soloff  1 , Jessica Tarabay  2 , Jay Varkey  3 , Andrew S Webster  3 , Daniela Weiskopf  4 , Daniel B Weissman  5 , Yongxian Xu  5 , Jesse J Waggoner  5 , Katia Koelle  5 , Nadine Rouphael  5 , Stephanie M Pouch  5 , Anne Piantadosi  5
Affiliations

SARS-CoV-2 Evolution and Immune Escape in Immunocompromised Patients

Erin M Scherer et al. N Engl J Med. .
No abstract available

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Figures

Figure 1
Figure 1. Neutralizing-Antibody Titers, Effector T-Cell Responses, and Spike Mutations in Five Immunocompromised Patients.
Panel A shows neutralizing-antibody titers in patient serum against Wuhan-Hu-1, the reference SARS-CoV-2 pseudovirus, at various time points after infection. These titers represent the reciprocal serum dilution at which half-maximal pseudovirus neutralization was observed. Data show the geometric means of two to five independent experiments; 𝙸 bars indicate standard deviations. The dotted line represents the lower limit of detection. Panels B and C show background-subtracted frequencies of CD4+ or CD8+ T cells expressing CD154, interferon-γ, tumor necrosis factor (TNF), or interleukin-2 as a percentage of non-naive (i.e., effector or memory) cells in response to stimulation of peripheral-blood mononuclear cells with a peptide megapool containing 15-mers from the spike open reading frame (ORF) and a peptide megapool containing predicted CD8+ T-cell epitopes from ORFs including spike, respectively. Frequencies were determined by flow cytometry in Patients 4 and 5, as well as in a healthy control donor (HC2) and two age-matched patients hospitalized with Covid-19 (Covid 1 and 2). Panel D shows mutations in the gene encoding the SARS-CoV-2 spike protein as compared with the Wuhan-Hu-1 strain, according to patient identifier and time point. Shading denotes mutation frequency. For each mutation, the observed variant nucleotide is listed above the plot and the amino acid mutation is listed below the plot.
See this image and copyright information in PMC

References

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