Targeted for destruction: degradation of singlet oxygen-damaged chloroplasts

Abstract

Photosynthesis is an essential process that plants must regulate to survive in dynamic environments. Thus, chloroplasts (the sites of photosynthesis in plant and algae cells) use multiple signaling mechanisms to report their health to the cell. Such signals are poorly understood but often involve reactive oxygen species (ROS) produced from the photosynthetic light reactions. One ROS, singlet oxygen (¹O₂), can signal to initiate chloroplast degradation, but the cellular machinery involved in identifying and degrading damaged chloroplasts (i.e., chloroplast quality control pathways) is unknown. To provide mechanistic insight into these pathways, two recent studies have investigated degrading chloroplasts in the Arabidopsis thaliana¹O₂ over-producing plastid ferrochelatase two (fc2) mutant. First, a structural analysis of degrading chloroplasts was performed with electron microscopy, which demonstrated that damaged chloroplasts can protrude into the central vacuole compartment with structures reminiscent of fission-type microautophagy. ¹O₂-stressed chloroplasts swelled before these interactions, which may be a mechanism for their selective degradation. Second, the roles of autophagosomes and canonical autophagy (macroautophagy) were shown to be dispensable for ¹O₂-initiated chloroplast degradation. Instead, putative fission-type microautophagy genes were induced by chloroplast ¹O₂. Here, we discuss how these studies implicate this poorly understood cellular degradation pathway in the dismantling of ¹O₂-damaged chloroplasts.

Keywords: Autophagy; cellular degradation; chloroplast; photosynthesis; reactive oxygen species; signaling.

Figure 1.

Singlet oxygen-induced selective chloroplast degradation.

Figure 2.

Models for chloroplast quality control pathways.