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. 2022 May 29:2022:7761852.
doi: 10.1155/2022/7761852. eCollection 2022.

Analysis of the Underlying Mechanism of the Jiu Wei Zhen Xin Formula for Treating Generalized Anxiety Disorder Based on Network Pharmacology of Traditional Chinese Medicine

Heng Shao  1   2 , Quan Gan  3 , Zhuangfei Chen  3 , Shasha Zhu  2   4 , Yanqing Zhu  2   4
Affiliations

Analysis of the Underlying Mechanism of the Jiu Wei Zhen Xin Formula for Treating Generalized Anxiety Disorder Based on Network Pharmacology of Traditional Chinese Medicine

Heng Shao et al. Evid Based Complement Alternat Med. .

Abstract

Currently, there are many effective pharmacological treatments for generalized anxiety disorder (GAD), formulated herbal granule is also an alternative way. Our research intends to construct a pharmacological network on genetic targets and pathways between Jiu Wei Zhen Xin Formula (JWZXF) and GAD. Through the TCMSP database, we collected the active ingredients of JWZXF and potential targets of the active ingredients. The GAD-related proteins collected from GeneCards database and DisGeNET database were combined. Component-target protein networks were constructed and visualized using Cytoscape 3.8.2 software to comprehensively clarify the relationships between ingredients, components, and targets. The intersection targets were imported into the STRING database, and the protein-protein interaction (PPI) network was constructed. We constructed and analyzed the visualized "drug-target-disease" network. Gene Ontology (GO) enrichment together with Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis were conducted on the common target through R language. Forty-one effective components and 106 potential targets of JWZXF were found. There were top ten hub genes and multiple important signaling pathways involved in the treatment of GAD with the JWZXF. This study expounded the pharmacological actions and molecular mechanisms of the JWZXF in treating GAD from a holistic perspective. The potential pharmacological effects of the JWZXF are closely related to regulation because not only does it comprehensively analyze the possible mechanism of JWZXF treatment of GAD but it can also facilitate further in-depth research and provide a theoretical basis for the clinical expansion of its application.

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Conflict of interest statement

The authors declare that there are no conflicts of interest regarding the publication of this paper.

Figures

Figure 1
Figure 1
Component-target protein network. The hexagons represent the 41 candidate compounds in the Jiu Wei Zhen Xin formula (JWZXF). The blue diamond represents the genetic names of the target proteins of the nine herbs found by text mining.
Figure 2
Figure 2
PPI network of JWZXF in the treatment of GAD-related proteins (a) and MCODE analysis of the PPI network and their corresponding GO biological processes (b).
Figure 3
Figure 3
Top 10 hub genes with their neighboring gene interactions. The blue circles are the neighbor gene, red and yellow circles are the hub gene, and the color changes from dark to light according to the MCC score from high to low.
Figure 4
Figure 4
KEGG enrichment analysis of the identified JWZXF in GAD treatment by DAVID database, ranked by an FDR value from low to high.
Figure 5
Figure 5
GO enrichment analysis of biological processes showing the top 15 items of 50 identified target proteins by DAVID database according to the FDR value sorted from small to large.
Figure 6
Figure 6
GO enrichment analysis of cellular components showing the top 15 items of 50 identified target proteins by DAVID database according to the FDR value sorted from small to large.
Figure 7
Figure 7
GO enrichment analysis of molecular functions showing the top 15 items of 50 identified target proteins by the DAVID database according to the FDR value sorted from small to large.
Figure 8
Figure 8
GABAergic synapse. The red rectangle represents the targets related to the component-target-pathway network, by Kanehisa Laboratories (https://www.kegg.jp/pathway/map04727).
Figure 9
Figure 9
Calcium signaling pathway. The red rectangle represents the targets related to the component-target-pathway network, by Kanehisa Laboratories (https://www.kegg.jp/entry/map04020).
See this image and copyright information in PMC

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References

    1. Maj M., Reed G. M. Bereavement-related depression in the DSM-5 and ICD-11. World Psychiatry . 2012;11(1):1–2. doi: 10.1016/j.wpsyc.2012.01.001. - DOI - PMC - PubMed
    1. American Psychiatric Association. Diagnostic and Statistical Manual of Mental disorders:DSM-5 . 5th. Arlington, TX, USA: American Psychiatric Association; 2013. pp. 222–226.
    1. Ruscio A. M., Hallion L. S., Lim C. C. W., et al. Cross-sectional comparison of the epidemiology of dsm-5 generalized anxiety disorder across the globe. JAMA Psychiatry . 2017;74(5):465–475. doi: 10.1001/jamapsychiatry.2017.0056. - DOI - PMC - PubMed
    1. Yu W., Singh S. S., Calhoun S., Zhang H., Zhao X., Yang F. Generalized anxiety disorder in urban China: prevalence, awareness, and disease burden. Journal of Affective Disorders . 2018;234:89–96. doi: 10.1016/j.jad.2018.02.012. - DOI - PubMed
    1. Chen T. R., Huang H. C., Hsu J. H., Ouyang W. C., Lin K. C. Pharmacological and psychological interventions for generalized anxiety disorder in adults: a network meta-analysis. Journal of Psychiatric Research . 2019;118:73–83. doi: 10.1016/j.jpsychires.2019.08.014. - DOI - PubMed

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