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. 2022 Jun 2;12(11):1904.
doi: 10.3390/nano12111904.

Biosynthesis of Platinum Nanoparticles with Cordyceps Flower Extract: Characterization, Antioxidant Activity and Antibacterial Activity

Ling Liu  1 , Yun Jing  2 , Ailing Guo  1 , Xiaojing Li  2 , Qun Li  1 , Wukang Liu  1 , Xinshuai Zhang  1
Affiliations

Biosynthesis of Platinum Nanoparticles with Cordyceps Flower Extract: Characterization, Antioxidant Activity and Antibacterial Activity

Ling Liu et al. Nanomaterials (Basel). .

Abstract

The aim of this work is to develop a green route for platinum nanoparticles (PtNPs) biosynthesized using Cordyceps flower extract and to evaluate their antioxidant activity and antibacterial activity. Different characterization techniques were utilized to characterize the biosynthetic PtNPs. The results showed that PtNPs were spherical particles covered with Cordyceps flower extract. The average particle size of PtNPs in Dynamic Light Scattering was 84.67 ± 5.28 nm, while that of PtNPs in Transmission Electron Microscope was 13.34 ± 4.06 nm. Antioxidant activity of PtNPs was evaluated by DPPH free radical scavenging ability test. The results showed that the antioxidant activity was positively correlated with the concentration of PtNPs, the DPPH scavenging efficiency of PtNPs (0.50-125.00 μg/mL) was 27.77-44.00%. In addition, the morphological changes of four kinds of bacteria (Escherichia coli, Salmonella typhimurium, Bacillus subtilis, Staphylococcus aureus) exposed to PtNPs were observed by scanning electron microscope. The results showed that the antibacterial activity of PtNPs against Gram-negative bacteria was stronger than that of Gram-positive bacteria.

Keywords: antibacterial activity; antioxidant activity; biosynthesis; characterization; cordyceps flower; platinum nanoparticles.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
UV-vis spectra of CE and PtNPs. The inset figure shows the color changes of PtNPs formation: (A) a mixture of Hexachloroplatinic acid and CE; (B) a mixture of the remaining CE and the synthetic PtNPs.
Figure 2
Figure 2
FT-IR spectra of CE and PtNPs.
Figure 3
Figure 3
Particle size distribution curve of PtNPs.
Figure 4
Figure 4
Zeta potential of PtNPs.
Figure 5
Figure 5
FE-SEM images of PtNPs: (a) the field of view at 10 μm; (b) the field of view at 1 μm.
Figure 6
Figure 6
TEM images of PtNPs: (a) the field of view at 200 nm; (b) the field of view at 10 nm.
Figure 7
Figure 7
EDS spectrum of PtNPs.
Figure 8
Figure 8
XRD spectrum of PtNPs.
Figure 9
Figure 9
DPPH scavenging activity of PtNPs and CE.
Figure 10
Figure 10
DPPH scavenging activity of standard Trolox.
Figure 11
Figure 11
Morphological changes of different bacteria before and after PtNPs treatment: (a–d) E. coli; (e–h) S. typhimurium; (i–l) B. subtilis; (m–p) S. aureus.
See this image and copyright information in PMC

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