Ultrastructural localization of beta-D-galactan in the nuclei of the myxomycete Physarum polycephalum

Abstract

The acellular slime mold Physarum polycephalum produces an extracellular sulfated and phosphorylated beta-D-galactan which was recently isolated from the nuclei of this organism. This polysaccharide has now been localized in the nuclei of P. polycephalum by electron microscopy using a specific "sandwich" technique: thin sections of P. polycephalum microplasmodia were incubated with the Ricinus communis lectin specific for D-galactose residues. The bound lectin was then localized with gold granules labeled with a galactose-terminated glycoprotein (desialylated ceruloplasmin). The galactan was found in the nuclei mainly associated with chromatin and, also, but to a smaller extent, in the cytoplasm and in some vacuoles. The specificity of the method was assessed by marking under the same condition the galactomannan present in the cell wall of the yeast Schizosaccharomyces pombe.