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Review
. 2022 Sep;38(5):e3281.
doi: 10.1002/btpr.3281. Epub 2022 Jun 24.

Immobilized enzymes as potent antibiofilm agent

Dibyajit Lahiri  1 , Moupriya Nag  1 , Ankita Dey  2 , Tanmay Sarkar  3 , Rina Rani Ray  4 , Maksim Rebezov  5   6   7 , Mohammad Ali Shariati  7   8 , Muthu Thiruvengadam  9 , Jesus Simal-Gandara  10
Affiliations
Review

Immobilized enzymes as potent antibiofilm agent

Dibyajit Lahiri et al. Biotechnol Prog. 2022 Sep.

Abstract

Biofilm has been a point of concern in hospitals and various industries. They not only cause various chronic infections but are also responsible for the degradation of various medical appliances. Since the last decade, various alternate strategies are being adopted to combat the biofilm formed on various biotic and abiotic surfaces. The use of enzymes as a potent anti-fouling agent is proved to be of utmost importance as the enzymes can inhibit biofilm formation in an eco-friendly and cost-effective way. The physical and chemical immobilization of the enzyme not only leads to the improvement of thermostability and reusability of the enzyme, but also gains better efficiency of biofilm removal. Immobilization of amylase, cellobiohydrolase, pectinase, subtilisin A and β-N-acetyl-glucosaminidase (DspB) are proved to be most effective in inhibition of biofilm formation and removal of matured biofilm than their free forms. Hence, these immobilized enzymes provide greater eradication of biofilm formed on various surfaces and are coming up to be the potent antibiofilm agent.

Keywords: antibiofilm; covalent crosslinking; enzyme; gel entrapment; immobilization.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

FIGURE 1
FIGURE 1
Mechanisms of enzyme immobilization
FIGURE 2
FIGURE 2
Mechanism of inhibition of biofilm by the immobilized enzyme
See this image and copyright information in PMC

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