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. 2022 Jun 3:14:100355.
doi: 10.1016/j.fochx.2022.100355. eCollection 2022 Jun 30.

TMT-based quantitative proteomics reveals protein biomarkers from cultured Pacific abalone (Haliotis discus hannai) in different regions

Yimu Luan  1   2 , Yonghui Dong  3 , Xuyuan Duan  1   2 , Xiuli Wang  4 , Yue Pang  1   2   5 , Qingwei Li  1   2   5 , Meng Gou  1   2   5
Affiliations

TMT-based quantitative proteomics reveals protein biomarkers from cultured Pacific abalone (Haliotis discus hannai) in different regions

Yimu Luan et al. Food Chem X. .

Abstract

Due to latitude, the growth cycle of abalone in southern China is significantly lower than that in the northern regions. Therefore, it often occurs merchants use southern abalone to disguise as northern abalone. This study aims to explore the differences in the muscle proteome of Pacific abalone (Haliotis discus hannai) in different regions. A total of 1,569 proteins were detected and 729 proteins were identified as differential abundance proteins (DAPs) in Haliotis discus hannai cultured in Northern (Liaoning Province) and Southern (Fujian Province) China. Bioinformatics analysis revealed and Western blot verified that fatty acid synthase, troponin I, calpain small subunit 1, and myosin light chain 6 are candidate biomarkers for abalone cultured in different regions. This study provides a deeper understanding of how to distinguish which region abalone is harvested from to improve abalone quality controls, and prevent food fraud.

Keywords: Haliotis discus hannai; Protein biomarker; Proteomics; TMT.

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Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Fig. 1
Fig. 1
Experimental design and workflow of this experiment.
Fig. 2
Fig. 2
Results of proteome analysis. (A) Basic information for protein identification. (B) Length distribution of identified peptides. (C) Score plot of principal component analysis (PCA) of Dalian abalone (N) and Fujian abalone (S). (D) Differential protein statistics chart. (E) Differential protein volcano map.
Fig. 3
Fig. 3
Gene ontology (GO) categorize of the DAPs in the Dalian abalone (N) and Fujian abalone (S).
Fig. 4
Fig. 4
The functions in different Q groups are drawn into heatmaps through hierarchical clustering. (A) Biological Process. (B) Cellular Component. (C) Molecular Function. (D) KEGG cluster analysis.
Fig. 5
Fig. 5
Biomarkers used to characterize abalone were confirmed by Western blot analysis. (A) Expression of FASN and TNNI1: Fatty acid synthase and Troponin I subunit in the S and N groups. (B) Expression of calpain small subunit 1 and myosin light chain 6 in groups S and N, GAPDH as a positive control.
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