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. 2022:2495:245-258.
doi: 10.1007/978-1-0716-2301-5_13.

Targeted Gene Editing in Porcine Germ Cells

Taylor Goldsmith  1 , Alla Bondareva  2 , Dennis Webster  1 , Anna Laura Voigt  2 , Lin Su  2 , Daniel F Carlson  1 , Ina Dobrinski  3
Affiliations

Targeted Gene Editing in Porcine Germ Cells

Taylor Goldsmith et al. Methods Mol Biol. 2022.

Abstract

As the genetic mutations driving human disease are identified, there is an increasing need for a biomedical model that can accurately represent the disease of interest and provide a platform for potential therapeutic testing. Pigs are a better model for human disease than rodents because of their genetic and physiological similarities to humans. However, current methods to generate porcine models are both technically challenging and expensive. Germline genetic modification through gene edited spermatogonia provides an effective alternative to how these models are developed. Here, we report an improved technique of gene editing in spermatogonia of pigs using CRISPR-Cas9 to generate different edits that reflect the genotypes of human diseases.

Keywords: CRISPR/Cas9; Gene editing; Pig; Spermatogonia.

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Figures

Figure 1:
Figure 1:
Schematic representation of the workflow involved in targeted gene editing of porcine spermatogonia.
See this image and copyright information in PMC

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