Structural characterization and antioxidant activity of Polygonatum sibiricum polysaccharides

Abstract

Polysaccharide is one of the main active components of Polygonatum sibiricum. For this study, P. sibiricum polysaccharides (PSP) were obtained through purification using DEAE-Cellulose52 and Sephacryl G-150 column chromatography. The obtained samples were named PSP1, PSP2 and PSP3. The PSP1 sample was found to have the highest content and the best solubility, and a subsequent. So, its structure and characterization were analyzed. The main sugar residue linkages were found to be → 1)-β-D-Fruf-(2 → 1)-β-D-Fruf-(2 → 1), 1 → -β-D-Fruf-(2 → 6)α-D-Glcp (1→, →4)-β-D-Manp-(1 → 4)-β-D-Manp-(1→ and →6)-β-D-Glcp-(1 → 4)-β-D-Manp-(1→ link existed. Branch chain analysis indicated →1,6)-β-D-Fruf-(2→, β-D-Fruf-(2→, →1,6)-β-D-Fruf-(2→, →6)-β-D- Fruf-(2→ link existed, and the link site was at position C-6. In vitro antioxidant activity tests showed that PSP1 had a certain scavenging effect on DPPH, hydroxyl radical, superoxide anion radical and a particular effect on the chelating ability of ferrous. This suggested that P. sibiricum polysaccharides may be a potential antioxidant.

Keywords: Antioxidant activity; Polygonatum sibiricum polysaccharides; Structure characterization.