Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Oct;57 Suppl 5(Suppl 5):90-93.
doi: 10.1111/rda.14179. Epub 2022 Jun 20.

Reproductive efficiency of sows inseminated at single dose fixed time with refrigerated, cryopreserved and encapsulated spermatozoa

Raúl Sánchez-Sánchez  1 , Sonia S Pérez-Garnelo  1 , Mercedes Martín Lluch  1 , Paloma de la Cruz  1 , Maria V Falceto  2 , Alejandro Córdova-Izquierdo  3 , Juan Grandía Torner  2 , Olga Mitjana  2 , Andrés E Suárez  2 , Teresa Montull  4 , Juan Grandía Ansó  4 , Trinidad Ansó  5 , Ernesto Gómez-Fidalgo  1
Affiliations

Reproductive efficiency of sows inseminated at single dose fixed time with refrigerated, cryopreserved and encapsulated spermatozoa

Raúl Sánchez-Sánchez et al. Reprod Domest Anim. 2022 Oct.

Abstract

The aim was to assess the reproductive efficiency of different techniques used to preserve spermatozoa in artificial insemination semen doses (AI-doses) by evaluating refrigeration at 15°C, cryopreservation and encapsulation. Forty-two hyperprolific sows were treated with buserelin and inseminated once at a single fixed time. The fertility rate, embryonic vesicles viability and the early embryonic mortality (arrested conceptuses) evaluated post-mortem at 24th day of pregnancy, were analysed in order to assess the effectiveness of each proposed technique. Results show an overall reduction on fertility using the three proposal sperm preservation techniques (69.27%, 60.00% and 78.75% for refrigerated, frozen-thawed and encapsulated AI-doses, respectively). Total number of embryonic vesicles was very similar among the three treatments; yet, the number of viable vesicles was numerically different among groups, and thus, embryonic viability was 79.25%, 80.0% and 87.15% for refrigerated, frozen-thawed and encapsulated AI-doses, respectively.

Keywords: Buserelin; cryopreserved semen; encapsulated spermatozoa; fixed-time insemination; refrigerated semen; semen preservation.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no competing interests.

Figures

FIGURE 1
FIGURE 1
Viable embryonic vesicle at D24 post‐AI
FIGURE 2
FIGURE 2
Non‐viable embryonic vesicles (arrested conceptuses) at D24 post‐AI
See this image and copyright information in PMC

References

    1. De Rensis, F. , & Kirkwood, R. N. (2016). Control of estrus and ovulation: Fertility to timed insemination of gilts and sows. Theriogenology, 86(6), 1460–1466. 10.1016/j.theriogenology.2016.04.089 - DOI - PubMed
    1. Driancourt, M. A. , Cox, P. , Rubion, S. , Harnois‐Milon, G. , Kemp, B. , & Soede, N. M. (2013). Induction of an LH surge and ovulation by buserelin (as Receptal) allows breeding of weaned sows with a single fixed‐time insemination. Theriogenology, 80(4), 391–399. 10.1016/j.theriogenology.2013.05.002 - DOI - PubMed
    1. Faustini, M. , Vigo, D. , Spinaci, M. , Galeati, G. , & Torre, M. L. (2012). Enhancing insemination performance in pigs through controlled release of encapsulated spermatozoa. Reproduction in Domestic Animals, 47, 353–358. 10.1111/j.1439-0531.2012.02098.x - DOI - PubMed
    1. Gil, J. , Jimenez, M. , Diaz, C. , Sanchez, R. , & Rillo, S. M. (1996). Swine AI with frozen boar semen in work routine. Reproduction in Domestic Animals, 31(1), 289–290.
    1. Knox, R. V. , Taibl, J. N. , Breen, S. M. , Swanson, M. E. , & Webel, S. K. (2014). Effects of altering the dose and timing of triptorelin when given as an intravaginal gel for advancing and synchronizing ovulation in weaned sows. Theriogenology, 82(3), 379–386. 10.1016/j.theriogenology.2014.04.020 - DOI - PubMed