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. 1978 Jul;14(3):477-88.
doi: 10.1016/0092-8674(78)90234-9.

Stereo immunofluorescence microscopy: I. Three-dimensional arrangement of microfilaments, microtubules and tonofilaments

M Osborn, T Born, H J Koitsch, K Weber

Stereo immunofluorescence microscopy: I. Three-dimensional arrangement of microfilaments, microtubules and tonofilaments

M Osborn et al. Cell. 1978 Jul.

Abstract

An easy manipulation of the commercial fluorescence microscope allows stereo pairs of pictures to be taken, which when examined with a stereo viewer, give a strong three-dimensional impression. The procedure is described in detail. Its use allows the documentation by immunofluorescence microscopy of the three-dimensional display and organization of microfilament bundles, microtubules and tonofilaments in some well characterized tissue culture cell lines.

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