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. 2022 May 15;14(5):3028-3036.
eCollection 2022.

The repair effect and mechanism of continuous passive motion on osteoarthritis in a rabbit model

Bingguang Zuo  1 , Zhipei Wang  2
Affiliations

The repair effect and mechanism of continuous passive motion on osteoarthritis in a rabbit model

Bingguang Zuo et al. Am J Transl Res. .

Abstract

Objective: To observe the effect of continuous passive motion (CPM) on osteoarthritis in a rabbit model and explore its mechanism.

Methods: Thirty healthy rabbits with a total of 60 knee joints were randomized into three groups. Group A had CPM for 8 h daily, starting on postoperative day 1 and free movement in the cage, group B received CPM for 2 h daily, starting on postoperative day 1 and free movement in the cage, and group C only had free movement in the cage. Mankin's score was used to compare the gross morphology of the rabbit's knee joint. Malondialdehyde (MDA) and superoxide dismutase (SOD) were measured by RT-PCR and western blot method before and after intervention.

Results: The Mankin's scores of rabbits in groups A and B were significantly lower than those in group C, and those in group A were lower than those in group B at week 4 and week 12 of intervention (P<0.05). At week 4 of the CPM intervention, the gross morphological scores were the highest in group A, followed by group B, and the lowest in group C (P<0.05). At week 12 of CPM intervention, the gross morphological scores of the knee joints in groups A and B were increased again, which were the highest in group A, followed by group B, and the lowest in group C (P<0.05). At week 12 of intervention, MDA levels in group A were lower than those in groups B and C, whereas SOD levels in group A were higher than those in groups B and C.

Conclusion: CPM can effectively improve the symptoms of knee osteoarthritis in rabbits and increase the mobility of the joints, and the mechanism may be related to the ability of CPM to reduce the overproduction of peroxide at the lesion site.

Keywords: Continuous passive motion; osteoarthritis malondialdehyde; superoxide dismutase.

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Conflict of interest statement

None.

Figures

Figure 1
Figure 1
Body weight of the rabbits in three groups. The differences among three groups before surgery, at the 4th week and at 12th week of CPM intervention were not statistically significant (P>0.05). n=5 rabbits (10 knees) each group.
Figure 2
Figure 2
HE staining of knee joint tissues in experimental rabbits. A-C: At 4 weeks after intervention, the staining of knee joint tissues in groups A, B, and C, respectively; D-F: At 12 weeks after intervention, the staining of knee joint tissues in groups A, B, and C, respectively.
Figure 3
Figure 3
Images of rabbit joints before and after intervention. A-C: At 4 weeks after intervention, the images of knee joint in groups A, B, and C, respectively; D-F: At 12 weeks after intervention, the images of knee joint in groups A, B, and C, respectively.
Figure 4
Figure 4
Changes in Mankin’s scores. A-C: Changes of Mankin’s scores over time in groups A, B, and C, respectively; D: Differences in Mankin’s scores among groups A, B and C at different time. #represents statistically significant difference between groups of the same index (P<0.05). n=5 rabbits (10 knees) each group.
Figure 5
Figure 5
Changes in the gross morphological scores of knee joints in each group. #represents statistically significant difference between groups of the same index. n=5 rabbits (10 knees) each group.
Figure 6
Figure 6
Changes in MDA. A-C: Changes of MDA levels over time in groups A, B, and C, respectively; D: Differences in MDA levels among groups A, B and C at different time. #represents statistically significant difference between groups of the same index (P<0.05). n=5 rabbits (10 knees) each group.
Figure 7
Figure 7
Changes in SOD before and after intervention. A-C: Changes of SOD levels over time in groups A, B, and C, respectively; D: Differences in SOD levels among groups A, B and C at different time. #represents statistically significant difference between groups of the same index (P<0.05). n=5 rabbits (10 knees) each group.
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