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Review
. 2022 Aug;22(15-16):e2100394.
doi: 10.1002/pmic.202100394. Epub 2022 Jun 26.

Advances in ionisation techniques for mass spectrometry-based omics research

Bob Challen  1 , Rainer Cramer  1
Affiliations
Review

Advances in ionisation techniques for mass spectrometry-based omics research

Bob Challen et al. Proteomics. 2022 Aug.

Abstract

Omics analysis by mass spectrometry (MS) is a vast field, with proteomics, metabolomics and lipidomics dominating recent research by exploiting biological MS ionisation techniques. Traditional MS ionisation techniques such as electrospray ionisation have limitations in analyte-specific sensitivity, modes of sampling and throughput, leading to many researchers investigating new ionisation methods for omics research. In this review, we examine the current landscape of these new ionisation techniques, divided into the three groups of (electro)spray-based, laser-based and other miscellaneous ionisation techniques. Due to the wide range of new developments, this review can only provide a starting point for further reading on each ionisation technique, as each have unique benefits, often for specialised applications, which promise beneficial results for different areas in the omics world.

Keywords: DESI; REIMS; acoustic droplet ejection; ionisation techniques; liquid MALDI.

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References

REFERENCES

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