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Review
. 2022 May 31:13:924410.
doi: 10.3389/fmicb.2022.924410. eCollection 2022.

Improved Conventional and New Approaches in the Diagnosis of Tuberculosis

Baoyu Dong  1 , Zhiqun He  1 , Yuqing Li  2 , Xinyue Xu  1 , Chuan Wang  1 , Jumei Zeng  1
Affiliations
Review

Improved Conventional and New Approaches in the Diagnosis of Tuberculosis

Baoyu Dong et al. Front Microbiol. .

Abstract

Tuberculosis (TB) is a life-threatening infectious disease caused by Mycobacterium tuberculosis (M. tuberculosis). Timely diagnosis and effective treatment are essential in the control of TB. Conventional smear microscopy still has low sensitivity and is unable to reveal the drug resistance of this bacterium. The traditional culture-based diagnosis is time-consuming, since usually the results are available after 3-4 weeks. Molecular biology methods fail to differentiate live from dead M. tuberculosis, while diagnostic immunology methods fail to distinguish active from latent TB. In view of these limitations of the existing detection techniques, in addition to the continuous emergence of multidrug-resistant and extensively drug-resistant TB, in recent years there has been an increase in the demand for simple, rapid, accurate and economical point-of-care approaches. This review describes the development, evaluation, and implementation of conventional diagnostic methods for TB and the rapid new approaches for the detection of M. tuberculosis.

Keywords: Mycobacterium tuberculosis; diagnostic approaches; early detection; improved conventional methods; tuberculosis.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
Various diagnostic tools for TB point-of-care testing.
See this image and copyright information in PMC

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