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. 2023 Feb 8;76(3):e990-e994.
doi: 10.1093/cid/ciac445.

Tuberculosis Molecular Bacterial Load Assay Reveals Early Delayed Bacterial Killing in Patients With Relapse

Nyanda Elias Ntinginya  1 , Abhishek Bakuli  2   3 , Daniel Mapamba  1 , Wilber Sabiiti  4 , Gibson Kibiki  5 , Lilian Tina Minja  6 , Davis Kuchaka  7 , Klaus Reither  6   8   9 , Patrick Peter John Phillips  10 , Martin Johan Boeree  11 , Stephen H Gillespie  4 , Michael Hoelscher  2   3 , Norbert Heinrich  2   3 ; Pan African Consortium for the Evaluation of Antituberculosis Antibiotics (PanACEA) Consortium
Affiliations

Tuberculosis Molecular Bacterial Load Assay Reveals Early Delayed Bacterial Killing in Patients With Relapse

Nyanda Elias Ntinginya et al. Clin Infect Dis. .

Abstract

Bacterial killing in patients with tuberculosis (TB) relapse was compared to that in patients achieving cure, measured by TB molecular bacterial load assay (TB-MBLA) or mycobacteria growth indicator tube (MGIT) time to positivity (TTP). TB-MBLA in 4 relapsed patients was significantly different compared to 132 cured patients after 2 weeks of treatment; MGIT TTP showed a significant difference from week 8.

Keywords: biomarker; clinical trial; relapse; tuberclosis.

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Conflict of interest statement

Potential conflicts of interest. N. E. N., D. M., W. S., G. K., L. T. M., D. K., K. R., P. P. J. P., M. J. B., S. H. G., M. H., and N. H. report an EDCTP grant through the PanACEA Consortium. Additionally, S. H. G. received a UK Medical Research Council grant through his institution, and N. H. has received a research grant from Beckman Coulter and equipment from Cepheid, as research support to his institution. All other authors report no potential conflicts. All authors have submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest. Conflicts that the editors consider relevant to the content of the manuscript have been disclosed.

Figures

Figure 1.
Figure 1.
Quantitative results for bacterial load measured by tuberculosis molecular bacterial load assay over time (A, B), and for mycobacteria growth indicator tube time to positivity (TTP) during treatment (C, D), separated by patient outcome at 12 months postrandomization. Smoothed trend lines for the longitudinal profiles have been described for (log[MBL + 1]) and (log[TTP], days). The black dashed line denotes the regression fit of the marginal component of the linear mixed-effect model, accounting for a linear and quadratic time trend, which is different for the groups cured, relapse, or no outcome in consideration. Abbreviations: MBL, molecular bacterial load; MBLA, molecular bacterial load assay; TTD, time to detection; TTP, time to positivity.
See this image and copyright information in PMC

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