Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1978;23(4):278-85.
doi: 10.1007/BF02876681.

Application of R-plasmid DNA's from Escherichia coli minicells in genetic transformation

J Nesvera, J Hochmannová, J Stokrová

Application of R-plasmid DNA's from Escherichia coli minicells in genetic transformation

J Nesvera et al. Folia Microbiol (Praha). 1978.

Abstract

Components of minicell lyzates of Escherichia coli P678.54 (R1 drd 19) and escherichia coli P678.54 (R6K) were visualized in an electron microscope and used for the transformation of Escherichia coli JC7623. The frequency of the resulting transformants (of the order of 10(-6)%) was not appreciably influenced by the manner of lyzate preparation. The presence of covalently closed circular DNA was detected in two different transformants using radioisotopes, thus demonstrating an autonomous existence of R1 drd 19 or R6K plasmids in tested transformants. This finding corresponds with the results of their genetic analysis.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Mol Gen Genet. 1975 Sep 15;140(1):39-50 - PubMed
    1. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 - PubMed
    1. Virology. 1959 Oct;9:260-74 - PubMed
    1. J Bacteriol. 1973 Nov;116(2):1064-6 - PubMed
    1. J Bacteriol. 1970 Oct;104(1):34-44 - PubMed