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. 2023 Jul;17(3):881-888.
doi: 10.1055/s-0042-1748529. Epub 2022 Jun 21.

Gingival Mesenchymal Stem Cells Metabolite Decreasing TRAP, NFATc1, and Sclerostin Expression in LPS-Associated Inflammatory Osteolysis In Vivo

Alexander Patera Nugraha  1   2 , Nastiti Faradilla Ramadhani  1   3 , Wibi Riawan  4 , Igo Syaiful Ihsan  5 , Diah Savitri Ernawati  6 , Rini Devijanti Ridwan  7 , Ida Bagus Narmada  1   2 , Tania Saskianti  8 , Fianza Rezkita  9 , Andari Sarasati  9 , Tengku Natasha Eleena Binti Tengku Ahmad Noor  10 , Bilqis Inayatillah  11 , Albertus Putera Nugraha  12 , Florentina Joestandari  13
Affiliations

Gingival Mesenchymal Stem Cells Metabolite Decreasing TRAP, NFATc1, and Sclerostin Expression in LPS-Associated Inflammatory Osteolysis In Vivo

Alexander Patera Nugraha et al. Eur J Dent. 2023 Jul.

Abstract

Objective: Bone is a dynamic tissue that undergoes remodeling. During bone remodeling, there are transcription factors such as nuclear factor-activated T cells-1 (NFATc1), sclerostin, and tartrate-resistant acid phosphatase (TRAP) that are released for bone resorption. Metabolite from gingival mesenchymal stem cells (GMSCs) has the ability to activate proliferation, migration, immunomodulation, and tissue regeneration of bone cells and tissues. Furthermore, the aim of this study is to investigate the metabolite of GMSCs' effect on expression of NFATc1, TRAP, and sclerostin in calvaria bone resorption of Wistar rats.

Materials and methods: Twenty male healthy Wistar rats (Rattus norvegicus), 1 to 2 months old, 250 to 300 g body were divided into four groups, namely group 1 (G1): 100 µg phosphate-buffered saline day 1 to 7; group 2 (G2): 100 μg lipopolysaccharide (LPS) day 1 to 7; group 3 (G3): 100 μg LPS + 100 μg GMSCs metabolite day 1 to 7; and group 4 (G4): 100 μg GMSCs metabolite day 1 to 7. Escherichia coli LPS was used to induce inflammatory osteolysis on the calvaria with subcutaneous injection. GMSCs metabolite was collected after passage 4 to 5, then injected subcutaneously on the calvaria. All samples were sacrificed on the day 8 through cervical dislocation. The expression of TRAP, NFATc1, and sclerostin of osteoclast in the calvaria was observed with 1,000× magnification.

Statistical analysis: One-way analysis of variance and Tukey honest significant different were conducted to analyze differences between groups (p < 0.05).

Results: The administration of GMSCs metabolite can significantly decrease TRAP, NFATc1, and sclerostin expression (p < 0.05) in LPS-associated inflammatory osteolysis calvaria in Wistar rats (R. norvegicus). There were significantly different TRAP, NFATc1, and sclerostin expressions between groups (p < 0.05).

Conclusion: GMSCs metabolite decrease TRAP, NFATc1, and sclerostin expression in LPS-associated osteolysis calvaria in Wistar rats (R. norvegicus) as documented immunohistochemically.

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Conflict of interest statement

None declared.

Figures

Fig. 1
Fig. 1
( A ) The (brown color) of tartrate-resistant acid phosphatase (TRAP) in osteoclasts in the calvaria in each group (black arrow) were observed by means of light inverted microscope at 100 × , 400 × , and 1,000× magnification. ( B ) The TRAP expression number in osteoclast in each group (* significant between groups [ p  < 0.05]).
Fig. 2
Fig. 2
( A ) The positive expression (brown color) of nuclear factor-activated T cells c1 (NFATc1) in osteoclasts in the calvaria in each group (black arrow) were observed by means of light inverted microscope at 100 × , 400 × , and 1,000× magnification. ( B ) The NFATc1 expression number in osteoclast in each group (* significant between groups [ p  < 0.05]).
Fig. 3
Fig. 3
( A ) The (brown color) of sclerostin in osteoclasts in the calvaria in each group (black arrow) were observed by means of light inverted microscope at 100 × , 400 × , and 1,000× magnification. ( B ) The sclerostin expression number in osteoclast in each group (* significant between groups [ p  < 0.05]).
See this image and copyright information in PMC

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