Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022:2505:301-315.
doi: 10.1007/978-1-0716-2349-7_22.

Agrobacterium-Mediated in Planta Transformation in Periwinkle

Dikki Pedenla Bomzan  1   2 , H B Shilpashree  1 , Dinesh A Nagegowda  3   4
Affiliations

Agrobacterium-Mediated in Planta Transformation in Periwinkle

Dikki Pedenla Bomzan et al. Methods Mol Biol. 2022.

Abstract

Madagascar periwinkle (Catharanthus roseus, family Apocynaceae) is a reservoir of more than 130 monoterpene indole alkaloids (MIAs) including the famous anti-neoplastic dimeric MIAs vinblastine and vincristine, and anti-hypertensive monomeric MIAs ajmalicine and serpentine. Understanding the biosynthetic steps and regulatory factors leading to the formation of MIAs is crucial for rational engineering to achieve targeted enhancement of different MIAs. Due to its highly recalcitrant nature, C. roseus is considered genetically non-tractable for transformation at the whole-plant level. Though few reports have demonstrated tissue culture-mediated regeneration and transformation of C. roseus at whole-plant level recently, the efficiency and reproducibility of these protocols have been a major challenge. To overcome this, we have developed a tissue-culture-independent Agrobacterium-mediated in planta transformation method in C. roseus. Using this method, we were able to efficiently generate stable transgenic plants without relying on the cumbersome methods of tissue-culture regeneration and transformation. Moreover, the transformed plants obtained through this in planta method exhibited stability in subsequent generations. Our method is useful not only for the elucidation of biosynthetic and regulatory steps involved in MIA formation through transgenic plant approach but also for metabolic engineering at the whole-plant level in C. roseus.

Keywords: Catharanthus roseus; In planta transformation; Monoterpene indole alkaloids; Plant secondary metabolism; Transgenics.

PubMed Disclaimer

References

    1. Kellner F, Kim J, Clavijo BJ, Hamilton JP, Childs KL, Vaillancourt B, Cepela J, Habermann M, Steuernagel B, Clissold L, McLay K (2015) Genome-guided investigation of plant natural product biosynthesis. Plant J 82(4):680–692 - DOI
    1. Zhao L, Sander GW, Shanks JV (2013) Perspectives of the metabolic engineering of terpenoid indole alkaloids in Catharanthus roseus hairy roots. Adv Biochem Eng Biotechnol 134:23–54 - PubMed
    1. Zhu X, Zeng X, Sun C, Chen S (2014) Biosynthetic pathway of terpenoid indole alkaloids in Catharanthus roseus. Front Med 8(3):285–293 - DOI
    1. Sottomayor M, De Pinto MC, Salema R, DiCosmo F, Pedreoo MA, Ros Barcelo A (1996) The vacuolar localization of a basic peroxidase isoenzyme responsible for the synthesis of α-31, 41-anhydrovinblastine in Catharanthus roseus (L.) G. Don Leaves. Plant Cell Environ 19(6):761–767 - DOI
    1. Costa MM, Hilliou F, Duarte P, Pereira LG, Almeida I, Leech M, Memelink J, Barceló AR, Sottomayor M (2008) Molecular cloning and characterization of a vacuolar class III peroxidase involved in the metabolism of anticancer alkaloids in Catharanthus roseus. Plant Physiol 146(2):403–417 - DOI

Publication types

MeSH terms

LinkOut - more resources